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TranscriptomicsProteomic profiling by array of yeast E2Bmin, mRNA and total RNA on fuctional protein microarrays to identify RNA-binding proteins
Application of different sorts of RNAs (E2Bmin, mRNA, total RNA) on functional protein micorarrays to identify RNA-binding proteins.
ORGANISM(S): Saccharomyces cerevisiae 
2011-09-01 | E-MEXP-2897 | biostudies-arrayexpress
ProteomicsmRNA binding - Insights into RNA biology from an atlas of mammalian mRNA-binding proteins
RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed "interactome capture," to define the mRNA interactome of proliferating hum...
ORGANISM(S): Homo sapiens (Human) 
2015-09-11 | PXD002880 | Pride
Mrna binding 
MetabolomicsJejunal inflammatory cytokines, barrier proteins and microbiome-metabolome responses to early supplementary feeding of Bamei suckling piglets

BACKGROUND: Dietary intervention has been reported to improve intestinal health. The intestinal microbiota of newborn animals plays a fundamental role in the development of intestinal function and the innate immune system. However, little is currently known about dietary interven...

2020-06-05 | MTBLS1698 | MetaboLights
OtherInsights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Data from ProteomeXchange, PXD ID: PXD002880. Experiment: KS_A4_3, file: folder summary. Published as part of Cell. 2012 Jun 8;149(6):1393-406 . From the Abstract: {{i}} RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV cro...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM11210041750 | GPMDB
Resource Reanalysis 
TranscriptomicsIntegrative regulatory mapping indicates that the RNA-binding protein HuR (ELAVL1) couples pre-mRNA processing and mRNA stability [sequence data]
RNA-binding proteins coordinate the fates of multiple RNAs, but the principles underlying these global interactions remain poorly understood. We elucidated regulatory mechanisms of the RNA-binding protein HuR, by integrating data from diverse high-throughput targeting technologies, specifically PAR-...
ORGANISM(S): Homo sapiens 
2011-08-19 | E-GEOD-29779 | biostudies-arrayexpress
ProteomicsYBX3 is a versatile RNA-binding protein that controls amino acid import by regulating SLC mRNA abundance
Summary: Amino acids (AA) support protein synthesis, and thus cell growth and proliferation. Specialized transporters mediate AA exchange across membranes and their regulation is critical for AA homeostasis. Here, we report that the DNA- and RNA-binding protein YBX3 regulates the expression of AA t...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-11-06 | MSV000083108 | MassIVE
ProteomicsHeLa mRNA interactome
Pull-down of poly(A)-mRNA cross linked proteins using two cross-linking methods (conventional cross-linking and PAR-cross-linking) to identify all mRNA-binding proteins (GO:0003729). The provided data is quantitative proteomic data for comparison of cross-linking and control samples.
ORGANISM(S): Homo sapiens 
2012-08-14 | E-MTAB-869 | biostudies-arrayexpress
ProteomicsmRNA-binding proteins of Leishmania donovani LC-MSMS
Leishmania are unicellular eukaryotes responsible for leishmaniasis in humans. Like other trypanosomatids, leishmania regulate protein coding gene expression almost exclusively at the post-transcriptional level with the help of RNA binding proteins (RBPs). Due to the presence of polycystronic trans...
ORGANISM(S): Leishmania donovani 
2017-02-08 | PXD005601 | Pride
Lc-msms Mrna-binding proteins Leishmania donovani Interactome capture 
TranscriptomicsPervasive and dynamic protein binding sites of the mRNA transcriptome in Saccharomyces cerevisiae
Protein-RNA interactions are integral components of nearly every aspect of biology including regulation of gene expression, assembly of cellular architectures, and pathogenesis of human diseases. However, studies in the past few decades have only uncovered a small fraction of the vast landscape of t...
ORGANISM(S): Saccharomyces cerevisiae 
2013-01-29 | E-GEOD-43747 | biostudies-arrayexpress
TranscriptomicsA quantitative analysis of CLIP methods for identifying binding sites of RNA-binding proteins (mRNA-seq)
Crosslinking and immunoprecipitation (CLIP) is increasingly used to map transcriptome-wide binding sites of RNA-binding proteins (RBPs). We developed a method for CLIP data analysis and applied it to compare 254 nm CLIP with PAR-CLIP, which involves crosslinking of photoreactive nucleotides with 365...
ORGANISM(S): Homo sapiens 
2011-05-15 | E-GEOD-28864 | biostudies-arrayexpress
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