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ProteomicsTop-down Proteomics of Endogenous Membrane Proteins Enabled by A Cloud Point Enrichment and Multidimensional Liquid Chromatography-Mass Spectrometry Top-down Proteomics of Endogenous Membrane Proteins Enabled by A Cloud Point Enrichment and Multidimensional Liquid Chromatography-Mass Spectrometry
Top-down proteomics has emerged as a powerful strategy to characterize proteins in biological systems. Nevertheless, the analysis of endogenous membrane proteins is challenging due to their low solubility, low abundance, and the complexity of the membrane subproteome. Here, we report a simple, effec...
ORGANISM(S): Homo sapiens (Human) 
2020-11-30 | PXD019368 | Pride
Membrane protein Top-down proteomics Cloud point 
ProteomicsAutomating Multidimensional Liquid Chromatography, Ion Mobility Spectrometry and Mass Spectrometry Data Analyses Using Skyline
Recent advances in ion mobility spectrometry (IMS) have illustrated its power in the structural characteristics of a molecule, especially when coupled with other separations dimensions such as liquid chromatography (LC) and mass spectrometry (MS). However, these three separation techniques together ...
ORGANISM(S): Bos Taurus Saccharomyces Cerevisiae 
PXD010650 | panorama
ProteomicsProteome analysis by charge state-selective separation of peptides: a multidimensional approach
Multidimensional peptide fractionation is widely used in proteomics to reduce the complexity of peptide mixtures prior to mass spectrometric analysis. Here, we describe the sequential use of strong cation exchange and reversed phase liquid chromatography in both basic and acidic pH buffers for separ...
ORGANISM(S): Mus musculus (Mouse) 
2013-11-27 | PXD000210 | Pride
Lc-msms Rp-hplc basic ph Mouse Scx 
UnknownMultidimensional persistence in biomolecular data.
Not available
S-EPMC4485576 | biostudies-literature
UnknownA Multidimensional Characterization of E3 Ubiquitin Ligase and Substrate Interaction Network.
Not available
S-EPMC6557761 | biostudies-literature
UnknownStereochemical Heterogeneity Analysis of Polylactides by Multidimensional Liquid Chromatography.
Not available
S-EPMC10955512 | biostudies-literature
ProteomicsMultidimensional separation schemes enhance the identification and molecular characterization of low molecular weight proteomes and short open reading frame encoded peptides in top-down proteomics
In the present work we developed a sample preparation approach for the combined bottom-up and top-down proteomics analysis of small open reading frame encoded proteins (SEP). Key improvements were made by the application of solid phase extraction (SPE) supported enrichment of LMW proteins, followed ...
ORGANISM(S): Methanosarcina mazei Go1 
2021-11-02 | PXD019792 | Pride
Microprotein Sep 
UnknownPrediction of Thermal Modulated Comprehensive Two-Dimensional Gas Chromatographic Separation Using a Modular, Graph-Based Simulation Platform.
Not available
S-EPMC12824439 | biostudies-literature
UnknownExcitonic structure and charge separation in the heliobacterial reaction center probed by multispectral multidimensional spectroscopy.
Not available
S-EPMC8121816 | biostudies-literature
UnknownFunctionalized boron nitride membranes with ultrafast solvent transport performance for molecular separation.
Not available
S-EPMC5954095 | biostudies-literature
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