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MetabolomicsDissection of two routes to naïve pluripotency using different kinase inhibitors
Embryonic stem cells (ESCs) can be maintained in the naïve state through inhibition of Mek1/2 and Gsk3 (2i). A relevant effect of 2i is the inhibition of Cdk8/19, which are negative regulators of the Mediator complex, responsible for the activity of enhancers. Inhibition of Cdk8/19 (Cdk8/19i) stimul...
2021-02-10 | MTBLS301 | MetaboLights
ProteomicsA High-Resolution Proteomic Atlas of Extracellular Vesicles and Non-Vesicular Particles
Extracellular vesicles (EVs) are key mediators of intercellular communication and promising biomarkers. However, their molecular characterization remains challenging due to the heterogeneity of EV subtypes and co-isolated nanoparticles. Here, leveraging protein correlation profiling along density gr...
ORGANISM(S): Homo sapiens (Human) 
2025-12-30 | PXD062547 | Pride
Density gradient Proteomics Extra-cellular vesicles Biofluids Cancer cells 
ProteomicsA High-Resolution Proteomic Atlas of Extracellular Vesicles and Non-Vesicular Particles
Extracellular vesicles (EVs) are key mediators of intercellular communication and promising biomarkers. However, their molecular characterization remains challenging due to the heterogeneity of EV subtypes and co-isolated nanoparticles. Here, leveraging protein correlation profiling along density gr...
ORGANISM(S): Homo sapiens (Human) 
2025-12-30 | PXD062477 | Pride
Density gradient Proteomics Extra-cellular vesicles Biofluids Cancer cells 
ProteomicsUse of lymph-circulating extracellular vesicles as surrogates of melanoma progression markers and BRAFV600E mutation.
Liquid biopsies are becoming imperative on early patient diagnosis, prognosis and evaluation of residual disease. The use of circulating extracellular vesicles (EVs) as surrogate markers of tumor progression could be a powerful tool in the clinical setting. EVs in plasma have emerged as a non-invasi...
ORGANISM(S): Homo sapiens (Human) 
2021-02-26 | PXD009505 | Pride
Melanoma Braf Lymphatic fluid Exosomes Metastasis 
ProteomicsA chemical screen reveals a connection between lipid metabolism and protein translation
Cells were treated with the inhibitor gor 3h and 6h. Additionally, proteasome degradation was blocked with MG132 in the samples treteaed for 3h. DMSO treated samples were used as control. Three independent experiments were conducted
ORGANISM(S): Homo sapiens (Human) 
2021-04-19 | PXD017445 | Pride
Skii Tmt Translation Lipids 
ProteomicsProteomics of Secretomes and Exosomes in Melanoma
Exosomes were purified from several cell lines with different metastatic potential. Seecreted proteins were isolated from the same samples using the approach from Eichelbaum et al. Nat Biotechnol 2012
ORGANISM(S): Homo sapiens (Human) 
2017-06-29 | PXD002703 | Pride
Melanoma Proteomics Secretomes Metastasis Exosomes 
ProteomicsThrombocytopenia-associated mutations in MASTL deregulate global phosphorylation and actin cytoskeleton dynamics in platelets
MASTL, also known as Greatwall, was originally characterized in flies and Xenopus as a mitotic kinase involved in the maintenance of the mitotic state by inhibiting PP2A-B55 complexes. A specific mutation (E167D) in the human MASTL gene was simultaneously linked to autosomal dominant thrombocytopeni...
ORGANISM(S): Mus musculus (Mouse) 
2019-11-12 | PXD009398 | Pride
Platelets Kinase Cell cycle Mastl Phosphoproteomics 
ProteomicsSTAT3 labels a subpopulation of reactive astrocytes required for brain metastasis
The brain microenvironment imposes a particularly intense selective pressure on metastasis initiating cells, but successful metastasis bypass this control through mechanisms that are poorly understood. Reactive astrocytes are key components of this microenvironment, as they have been observed to con...
ORGANISM(S): Mus musculus (Mouse) 
2018-07-16 | PXD008956 | Pride
Astrocytes Brain metastasis Stat3 Secretome 
ProteomicsIdentification of brain metastasis vulnerabilities using METPlatform
Exclusion of cancer patients with brain metastases from clinical trials is a major cause of the limited therapeutic options available for secondary brain tumors. Here, we report a novel drug-screening platform (METPlatform) based on organotypic cultures that allows identifying anti-metastatic compou...
ORGANISM(S): Homo sapiens (Human) 
2022-03-17 | PXD020092 | Pride
Metplatform In situ proteomics Brain metastasis 
ProteomicsMASS SPECTROMETRY ROADMAPS TO NAIVE PLURIPOTENCY
Pluripotency can be maintained in the naïve state through manipulation of ERK and WNT signalling (2i), shielding embryonic stem cells (ESCs) from inductive cues. Alternatively, inhibiting CDK8/19 (CDK8/19i), a repressor of the Mediator co-activator complex, directly stimulates super-enhancer activit...
ORGANISM(S): Mus musculus (Mouse) 
2021-02-10 | PXD018694 | Pride
Phosphoproteomics Mouse stem cells Proteomics 
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