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ProteomicsLectin-based SP3 technology enables N-glycoproteomics analysis
We developed a glycosylation enrichment method based on lectin and single-pot, solid-phase-enhanced sample preparation (SP3) technology, termed lectin-based SP3 technology (LectinSP3). LectinSP3 immobilized lectin on the SP3 beads for N-glycopeptide enrichment. It could identify over 1,100 N-glycosy...
ORGANISM(S): Mus musculus (Mouse) 
2024-04-10 | PXD047610 | Pride
Testis Glycoproteomics Sp3 Oocyte N-glycosylation Lectin 
ProteomicsLectin-based affinity enrichment and characterization of N-glycoproteins from the human tear film by mass spectrometry
Glycosylation of proteins is one of the most common post-translational modification (PTM) and plays important regulatory functions in diverse biological processes such as protein stability or cell signaling. Accordingly, glycoproteins are also a consistent part of the human tear film proteome mainta...
ORGANISM(S): Homo sapiens (Human) Sus scrofa domesticus (domestic pig) 
2023-01-25 | PXD038280 | Pride
N-glycoproteins Mass spectrometry Pngase f Tear film Multi-lectin column 
ProteomicsDiscovery and validation of serum glycoprotein biomarker panel for detection of high grade serous ovarian cancer
We aimed to discover and validate a panel of serum biomarkers for high grade serous ovarian cancer (HGSOC) using our lectin-magnetic bead array-coupled proteomics platform. Serum from age-matched women with HGSOC, benign tumours or healthy controls were analysed in discovery (UKCTOCS, n=30 and UKOPS...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD032299 | Pride
Ovarian cancer screening Stl Glycosylation Lemba Lectin 
UnknownA synthetic lectin for O-linked beta-N-acetylglucosamine.
Not available
S-EPMC2835298 | biostudies-literature
ProteomicsDiscovery and validation of serum glycoprotein biomarker panel for detection of high grade serous ovarian cancer
We aimed to discover and validate a panel of serum biomarkers for high grade serous ovarian cancer (HGSOC) using our lectin-magnetic bead array-coupled proteomics platform. Serum from age-matched women with HGSOC, benign tumours or healthy controls were analysed in discovery (UKCTOCS, n=30 and UKOPS...
ORGANISM(S): Homo Sapiens 
2024-08-06 | PXD033108 | panorama
UnknownBivalent carbohydrate binding is required for biological activity of Clitocybe nebularis lectin (CNL), the N,N'-diacetyllactosediamine (GalNAcβ1-4GlcNAc, LacdiNAc)-specific lectin from basidiomycete C. nebularis.
Not available
S-EPMC3323013 | biostudies-literature
UnknownLectin Drug Conjugates Targeting High Mannose N-Glycans.
Not available
S-EPMC9738879 | biostudies-literature
UnknownDiscovery of a lectin domain that regulates enzyme activity in mouse N-acetylglucosaminyltransferase-IVa (MGAT4A).
Not available
S-EPMC9296478 | biostudies-literature
TranscriptomicsInflammatory gene expression in response to sub-lethal ricin exposure in Balb/c mice
The toxin ricin has been shown to cause inflammatory lung damage, leading to pulmonary oedema and, at higher doses, mortality. In order to understand the genetic basis of this inflammatory cascade a custom microarray platform (1509 genes) directed towards immune and inflammatory markers was used to ...
ORGANISM(S): Mus musculus 
2009-08-19 | E-GEOD-17431 | biostudies-arrayexpress
UnknownProbing N-glycoprotein microheterogeneity by lectin affinity purification-mass spectrometry analysis.
Not available
S-EPMC6524569 | biostudies-literature
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