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ProteomicsN-terminal acetylome analysis reveals an expanded substrate profile for the NatC N-terminal acetyltransferase complex in yeast
N-terminal acetylation is a conserved protein modification among eukaryotes, and the yeast Saccharomyces cerevisiae is a valuable model system for studying this modification. The enzymes responsible for the bulk of protein N-terminal acetylation in S. cerevisiae are the N-terminal acetyltransferases...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-01-26 | PXD039544 | Pride
Natc N-terminal acetylation N-alpha-acetyltransferase 30 (naa30) Saccharomyces cerevisiae N-terminal acetylome N-terminal cofradic N-terminomics. 
ProteomicsN-terminal acetylation by NatB in Arabidopsis thaliana
N-terminal acetylation (NTA) is one of the most abundant protein modifications in eukaryotes and is catalysed in humans by seven N-acetyltransferases. In this study, we investigated the Arabidopsis thaliana NatB catalytic (NAA20) and auxiliary subunit (NAA25) and their influence on protein N-termina...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-11-21 | PXD016209 | Pride
Acetyltransferase N-acetylome N-terminal acetylation Quantitative proteomics Silpronaq Gap test 
ProteomicsMetabolic regulation of N-terminal acetylation in yeast
N-terminal (Nt) acetylation, catalyzed by N-terminal acetyltransferases (NATs), has emerged as an important co-translational modification in eukaryotes, and involves the transfer of the acetyl moiety from acetyl-CoA (Ac-CoA) to the α-amino group of a nascent polypeptide. Here, we report the first gl...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-09-07 | PXD004326 | Pride
Metabolism Acetyl coenzyme a Saccharomyces cerevisiae N-terminal acetylation N-terminomics 
TranscriptomicsTranscription profiling of S. cerevisiae H2B^3-32 mutants to investigate the role of hstone H2B N-terminal domain in genome wide transcription
Histone H2B was mutated to give H2B^3-32. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B N-terminal domain. Ex...
ORGANISM(S): Saccharomyces cerevisiae 
2008-06-12 | E-GEOD-3802 | biostudies-arrayexpress
ProteomicsPositional proteomics reveals differences in N-terminal proteoform stability.
To understand the impact of alternative translation initiation on a proteome, we performed the first large-scale study of protein turnover rates in which we distinguish between N-terminal proteoforms pointing to translation initiation events. Using pulsed SILAC combined with N-terminal COFRADIC we m...
ORGANISM(S): Homo sapiens (Human) 
2013-03-14 | PXD002091 | Pride
Nt-acetylation Protein stability Alternative translation initiation Initiator methionine processing Protein turnover N-terminal proteoform Human N-terminal cofradic 
ProteomicsGlobal acetylation profiling for multiple Arabidopsis thaliana plastid N-terminal acetyltransferases
Protein acetylation is a universally conserved modification occurring on N-termini (N-Terminal acetylation, NTA). Although recent reports indicate that NTA occur frequently in plant plastids, little is known about the machinery involved in plastid acetylation and why these modifications are that fre...
ORGANISM(S): Escherichia coli 
2020-07-13 | PXD016205 | Pride
Acetyltransferase N-acetylome N-terminal acetylation Quantitative proteomics Silpronaq Gap test 
ProteomicsAn OMICS perspective on N-terminal Methionine Excision
Excision of the N-terminal initiator methionine (iMet) from nascent peptide chains is an essential and omnipresent protein modification carried out by Methionine aminopetidases (MetAPs) and accounting for a major source of N-terminal proteoform diversity. While MetAP2 is known to be implicated in pr...
ORGANISM(S): Homo sapiens (Human) 
2018-01-10 | PXD006638 | Pride
Metap Metap1 Shotgun N-terminal proteomics Imet excision 
ProteomicsDeveloping a Novel and Integrative Method to Systematically Investigate the Stability of N-Terminal Proteoforms
Protein N-terminal proteoforms, which include both N-terminal modifications and sequence variation arising from processes such as proteolytic cleavage, are common in cells and have been reported to significantly impact protein stability. Systematically examining the correlation between these N-termi...
ORGANISM(S): Homo sapiens (Human) 
2025-08-21 | PXD057760 | Pride
Thermal proteome profiling N-terminal acetylation N-terminome Thermal stability difference Terminal amine isotopic labeling of substrates Tails Tpp N-terminal proteoform 
Proteomicsplasma membrane anchored NAA60 N-terminal acetylation profiling in Arabidopsis thaliana
N-terminal acetylation (NTA) is one of the most abundant protein modifications in eukaryotes and is catalysed in humans by seven N-acetyltransferases. AtNAA60 localizes to the plasma membrane in vivo by an α-helical membrane anchor at its C-terminus. In this study, we investigated the Arabidopsis th...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-07-15 | PXD016494 | Pride
Acetyltransferase N-acetylome N-terminal acetylation Quantitative proteomics Silpronaq 
ProteomicsAtNAA50 N-terminal acetylation profiling using A. thaliana naa50 KO mutants
N-terminal acetylation is one of the most abundant protein modifications in eukaryotes and is catalysed in humans by seven N-terminal acetyltransferases. AtNAA50 interreact with the NatA complex (NAA10 and NAA15) to form the NatE complex. In this study, we investigated the Arabidopsis thaliana N-ter...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-07-15 | PXD017770 | Pride
Acetyltransferase N-acetylome N-terminal acetylation Quantitative proteomics Silpronaq Gap test Encounter 
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