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To identify the proteins synthesized from long-lived mRNAs at early phase of germination, proteome of embryos at 0 DAI (dry embryos), 1 DAI with α-amanitin and 1 DAI without α-amanitin were analyzed by using LC-MS/MS.
ORGANISM(S): Oryza sativa (Rice) 
2019-08-13 | PXD013628 | Pride
To verify if CDKA;1 phosphorylates SWI1, recombinant proteins were prepared using E.coli system and in vitro kinase reactions were performed.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Escherichia coli 
2019-04-25 | PXD009959 | Pride
In order to identify the key factors that are crucial for non-host resistance against Bgh and are secreted depending on SYP4-mediated membrane trafficking, we have performed apoplastic fluid preparation of a series of mutants defective in the SYP4 pathway. This project aims to identify proteomic pro...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-01-11 | PXD009099 | Pride
Clock-generated biological rhythms provide an adaptive advantage to an organism which results in increased fitness and survival. To better elucidate the plant response to the circadian system, we surveyed protein oscillations in Arabidopsis seedlings under constant light. Using large-scale two-dimen...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-06-29 | PXD004276 | Pride
Lipids play crucial roles in plant-microbe interactions, functioning as structural components, signaling molecules, and microbe-associated molecular patterns (MAMPs); however, the mechanisms underlying lipid perception and signaling pathways in plants remain largely unknown. This study investigates ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-01-31 | PXD056788 | Pride
Genes encoding Arabidopsis subunit homologs of TFIIH kinase module were labelled with coding sequences of GFP and mCherry (green and red fluorescent proteins) using fast-track recombineering. To illustrate the applicability of recombineering for accelerating the isolation and identification of plant...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-08-01 | PXD013637 | Pride
Ustilago maydis is a biotrophic fungus that infects arial parts of maize, secreting effector proteins to facilitate host colonization and manipulate cellular processes, creating nutrient-rich environments for its growth and reproduction. Here, we identified three effectors (Hap1-3; hypertrophy-assoc...
ORGANISM(S): Zea mays (Maize) 
2026-01-28 | PXD057676 | Pride
Xylem vessels function in the long-distance conduction of water in land plants. The NAC transcription factor VASCULAR-RELATED NAC-DOMAIN7 (VND7) is a master regulator of xylem vessel cell differentiation in Arabidopsis (Arabidopsis thaliana). We previously isolated seiv (suppressor of ectopic xylem ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-10-17 | PXD048830 | Pride
Gene regulation of bacterial pathogens in the host is not comprehensively understood due to the difficulty in analyzing genome-wide mRNA and protein expression of bacteria during infection. Here, we jointly analyzed transcriptome and proteome of a foliar bacterial pathogen in plants. Bacterial trans...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-04-30 | PXD015839 | Pride
Several pathways conferring environmental flowering responses in Arabidopsis converge on developmental processes that mediate floral transition in the shoot apical meristem. Many characterized mutations disrupt these environmental responses, but downstream developmental processes have been more refr...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-03-02 | PXD016457 | Pride
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