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ProteomicsDIA proteomics of dried blood spot proteins by sodium carbonate
We developed a simple and sensitive analysis method for dried blood spot (DBS) proteins by sodium carbonate precipitation (SCP). We aimed to removing large amounts of hydrophilic blood proteins such as hemoglobin, albumin, fibrinogen and transferrin in DBS using a simple pretreatment with sodium car...
ORGANISM(S): Homo Sapiens (human) 
2020-01-20 | PXD017185 | JPOST Repository
ProteomicsLow-input proteomics using lauryl maltose neopentyl glycol
We developed a sample preparation method for low-input proteomics using lauryl maltose neopentyl glycol. Using the developed method, we performed proteome analysis of coIP samples.
ORGANISM(S): Homo Sapiens (human) 
2024-03-06 | PXD048100 | JPOST Repository
ProteomicsLow-input proteomics using lauryl maltose neopentyl glycol
We developed a sample preparation method for low-input proteomics using lauryl maltose neopentyl glycol. Using the developed method, we performed proteome analysis of 100ng of HEK293T proteins, coIP samples, and serum extracellular vesicles, and further challenged label-free single-cell proteomics o...
ORGANISM(S): Homo Sapiens (human) Bos Taurus 
2024-03-06 | PXD044605 | JPOST Repository
ProteomicsValidation of Thin-diaPASEF for maximizing proteome coverage
We optimized the parameters of CCS range, polygon scan region, ramp time, and isolation window width to achieve high-depth proteome analysis using the timsTOF HT. As a result, we developed Thin-diaPASEF, which was optimized with a CCS range of 0.7–1.3, a ramp time of 150 ms, an isolation window widt...
ORGANISM(S): Homo Sapiens (human) Cellular Organisms 
2025-08-10 | PXD061613 | JPOST Repository
ProteomicsDevelopment of non-targeted proteome analysis of dried blood spots using an automated protein extraction system (
In this study, we developed a simple protein extraction method of dried blood spots (DBS) that potentially meets the throughput required for newborn screening (NBS) and optimized non-targeted proteomic analysis using this method in combination with liquid chromatography coupled mass spectrometry in ...
ORGANISM(S): Homo Sapiens (human) 
2025-08-14 | PXD064701 | JPOST Repository
ProteomicsProteome analysis of serum proteins enriched by lectins
Serum proteins enriched by 37 different lectins were analyzed by LC-MS/MS. It was found that the enrichment process with Solanum tuberosum lectin (STL) and Lycopersicon esculentum lectin (LEL) detected many trace proteins in the serum. In addition, combinations of lectins were examined, and the comb...
ORGANISM(S): Homo Sapiens (human) Gallus Gallus Rattus Norvegicus Ovis Aries Cellular Organisms Capra Hircus Bos Taurus Mus Musculus (mouse) 
2024-02-23 | PXD047848 | JPOST Repository
ProteomicsProteome analysis of serum proteins enriched by STL, LEL and WGA
Serum proteins enriched by solanum tuberosum lectin (STL), lycopersicon esculentum lectin (LEL) and wheat germ agglutinin (WGA) were analyzed by LC-DIA-MS/MS using Q-Exactive HF-X (ThermoFisher Scientific).
ORGANISM(S): Homo Sapiens (human) 
2024-02-24 | PXD048327 | JPOST Repository
ProteomicsProteome analysis of serum proteins enriched by MOA and PSL1a
We conducted serum protein enrichment using Marasmius oreades agglutinin (MOA) and Polyporus squamosus lectin (PSL1a), followed by analysis using HF-X.
ORGANISM(S): Homo Sapiens (human) 
2025-04-30 | PXD051875 | JPOST Repository
TranscriptomicsCyclic tensile strain enhances the expression of Indian hedgehog and progression of ossification of the human cervical posterior longitudinal ligament
The initiation and/or progression of ossification of the posterior longitudinal ligament (OPLL) is associated with cyclic tensile strain, but the pathomechanism of OPLL remains unclear. Indian hedgehog (Ihh) and its related signaling are key factors in normal enchondral ossification. However, the r...
ORGANISM(S): Homo sapiens 
2016-06-22 | E-GEOD-83569 | biostudies-arrayexpress
ProteomicssJIA serum proteome profiling with DIA-MS
We performed a DIA-MS proteomic analysis of sera from systemic juvenile idiopathic arthritis with different activity phases using a high protein depletion process. We profiled the proteins in the sera that differed significantly in their activity phase.
ORGANISM(S): Homo Sapiens (human) 
2022-04-26 | PXD030413 | JPOST Repository
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