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To identify candidate genes unbiasedly, we employed biotinylation-based proximity proteomics of T-tubules in vivo. We generated a transgenic line carrying an Amph construct fused with miniTurbo (mnTb). We expressed either Amph-mnTb or mnTb alone in muscles, and larvae were reared in the presence or ...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
To identify candidate genes unbiasedly, we employed biotinylation-based proximity proteomics of T-tubules in vivo. We generated a transgenic line carrying an LUBEL construct fused with miniTurbo (mnTb). We expressed either LUBEL-mnTb or mnTb alone in muscles, and larvae were reared in the presence o...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
HA-LUBEL 1-872 or LacZ (control) was expressed under the control of Mef2-GAL4, and the progenies were raised at 25°C. The 3IL carcass fillet lysates were subjected to anti-HA immunoprecipitation. To identify potential binding partners, we performed immunoprecipitation-mass spectrometry (IP-MS) of LU...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
Xeno-free culture systems have expanded the application of human pluripotent stem cells (PSCs). Here we describe an improved method for the subculture of human PSCs. The revised method significantly facilitated the viability of human PSCs by lowering DNA damage and apoptosis, resulting in more effic...
ORGANISM(S): Homo Sapiens (human) 
Here, by combining transcriptome and proteome profiling, we identified 228 post-transcriptionally regulated genes with strict regulation at the protein level in PSCs. Among them, 84 genes were found necessary for the survival of several cell types including PSCs, and 20 genes were identified as spec...
ORGANISM(S): Homo Sapiens (human) 
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