NanoLuc-targeting PROTACs (NanoTACs) hijack the CRBN and VHL cullin-RING ligase complexes to trigger proteasomal degradation of substrates tagged with the 19 kDa luminescent NanoLuc tag. In order to validate the system and confirm selective degradation of the target protein and assess any potential ...
The ability to endow constitutively active proteins with synthetic allostery is a central objective of Synthetic Biology, mostly focused on the creation of protein biosensors – artificial sensing proteins with easily quantifiable outputs. Here we hypothesize that circular permutation of proteins inc...
