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UnknownpSNAP: Proteome-wide analysis of elongating nascent polypeptide chains.
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S-EPMC9218386 | biostudies-literature
UnknownA new strategy to uncover fragile X proteomic biomarkers using the nascent proteome of peripheral blood mononuclear cells (PBMCs).
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S-EPMC8313568 | biostudies-literature
ProteomicsNascent proteome analysis of proliferating and senescent BJ fibroblast treated with GC7
Analysis of proteomic changes in proliferating and senescent BJ fibroblast treated with eIF5A hypusination inhibitor GC7 for 12h.
ORGANISM(S): Homo sapiens (Human) 
2024-07-12 | PXD034334 | Pride
Gc7 Eif5a hypusination Nascent proteome 
ProteomicsCell-selective multiplexed bioorthogonal noncanonical amino acid tagging for versatile nascent proteome profiling
Cell-selective bioorthogonal noncanonical amino acid tagging (BONCAT) of newly synthesized proteins, catalyzed by engineered aminoacyl-tRNA synthetase (aaRSs), is a powerful technology for time-resolved characterization of the nascent proteome. Currently, cell-selective BONCAT studies in bacteria re...
ORGANISM(S): Klebsiella pneumoniae ATCC 43816 Escherichia coli Acinetobacter baumannii (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377) 
2026-04-11 | PXD067013 | Pride
Chemoproteomics Boncat Nascent proteins 
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains - pSNAP evaluation
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2023-02-07 | PXD024078 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains – IP input
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2022-02-09 | PXD024079 | JPOST Repository
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains – peptide pulldown
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Homo Sapiens (human) 
2022-05-28 | PXD026349 | JPOST Repository
ProteomicsCell type-specific metabolic labelling of nascent proteomes in vivo
Changes in protein expression drive both the acute responses and long-lasting adaptation of cells to internal and external stimuli. While advances in labelling strategies have made it possible to examine general proteome dynamics, it is still not possible to access the proteomes of specific cell typ...
ORGANISM(S): Mus musculus (Mouse) 
2017-11-10 | PXD007703 | Pride
Anl Boncat Azidonorleucine Nascent proteomes Funcat Metabolic labeling 
ProteomicsProteome-wide analysis of elongating nascent polypeptide chains - mouse primary neuron
Cellular global translation is often measured using ribosome profiling or quantitative mass spectrometry, but these methods do not provide direct information at the level of elongating nascent polypeptide chains (NPCs) and associated co-translational events. We combine quantitative proteomics, pulse...
ORGANISM(S): Mus Musculus (mouse) 
2023-02-07 | PXD024104 | JPOST Repository
Proteomicsnascent proteome analysis of HCT116 TP53 wild type and TP53 knockout cells treated with Neocarcinostatin (NCS) for 6h
In order to investigate the potential role of TP53 in regulating translation, HCT116 wild type and TP53 knockout cells were analyzed using both RNA sequencing, Ribosome sequencing and nascent proteome analysis. The cells were treated with 0.2 µg/ml Neocarcinostatin (NCS) to induce DNA damage and act...
ORGANISM(S): Homo sapiens (Human) 
2022-04-27 | PXD029512 | Pride
Tp53 Ncs Nascent proteome Metabolic labeling Dna damage 
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