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ProteomicsHDX-MS of CHIP wt and K30A mutant
HDX-MS was used to establish that conformational-inhibition-signals extended from the TPR-domain to the U-box of CHIP. This underscores inter-domain allosteric regulation of CHIP by the core molecular chaperones. Defining the chaperone-associated TPR-domain of CHIP as a manager of inter-domain commu...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2015-07-30 | MSV000079205 | MassIVE
ProteomicsQuantitative ChIP-MS analysis of histone modifications present on H3K4me1- and H3K4me3-enriched mono-nucleosomes purified from crosslinked IMR-90 cells
The INO80 complex is a chromatin remodeler that regulates DNA replication, repair, and transcription. Although the INO80 complex plays a crucial role in various chromatin-associated processes, the mechanism of its recruitment to specific genomic loci is not well understood. Here we used a native ChI...
ORGANISM(S): Homo sapiens (Human) 
2023-12-12 | PXD042224 | Pride
H3k4me3 H3k4me1 Histone ptms Chip-ms 
ProteomicsCTCF ChIP-MS from native chromatin in mouse embryonic stem cells (mESCs) and motor neurons (MNs)
To identify CTCF partners and co-localizing proteins from native chromatin in mESCs and MNs in endogenous conditions. The goal is to identify proteins within each sample with respect to wild-type control for each.
ORGANISM(S): Mus musculus (Mouse) 
2022-02-11 | PXD030452 | Pride
Mouse Native chromatin Ctcf chip-ms Ctcf partners Lc-ms/ms 
ProteomicsMultiphasic chip performance characterization using human thyroid cancer cell line SW1736 and human plasma
Development of an online peptide fractionation system comprising a multiphasic LC chip that integrates reversed phase and strong cation exchange chromatography upstream of the MS based on the multi-dimensional protein identification technology (mudPIT). Chips were assessed in terms of chromatographi...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000081545 | MassIVE
ProteomicsProteomic analysis of histone modifications present on nucleosomes bound by INO80 complex
The INO80 complex is a chromatin remodeler that regulates DNA replication, repair, and transcription. Although the INO80 complex plays a crucial role in various chromatin-associated processes, the mechanism of its recruitment to specific genomic loci is not well understood. Here we used a native ChI...
ORGANISM(S): Homo sapiens (Human) 
2023-12-10 | PXD042210 | Pride
Native chip-ms Ino80 Histone ptms 
ProteomicsLptM promotes oxidative maturation of the lipopolysaccharide translocon by substrate binding mimicry.
Insertion of lipopolysaccharide (LPS) into the outer membrane (OM) of Gram-negative bacteria is mediated by a druggable OM translocon consisting of a beta-barrel membrane protein, LptD, and a lipoprotein, LptE. The beta-barrel assembly machinery (BAM) assembles LptD together with LptE to form a plug...
ORGANISM(S): Escherichia coli 
2023-10-11 | PXD041774 | Pride
Lptde Native ms Hdx-ms Membrane protein Translocon 
ProteomicsStructural basis of gate dynamics at the outer membrane lipopolysaccharide holo-translocon
Gram-negative bacteria resist many harmful chemicals, protected by an asymmetric outer membrane (OM) containing lipopolysaccharide (LPS) in its external leaflet. Leaflet-selective assembly of LPS is an essential, yet mechanistically elusive process. This reaction is mediated by the OM LPS core trans...
ORGANISM(S): Escherichia coli 
2025-12-01 | PXD068376 | Pride
Lps translocon Native ms Gel digestion 
ProteomicsStreamlined Single-cell Proteomics by an Integrated Microfluidic Chip and Data-independent Acquisition Mass Spectrometry
Single-cell proteomics can reveal cellular phenotypic heterogeneity and cell-specific functional networks underlying biological processes. Here, we present a streamlined workflow combining microfluidic chips for all-in-one proteomic sample preparation and data-independent acquisition (DIA) mass spec...
ORGANISM(S): Homo Sapiens (human) Cellular Organisms 
2021-11-12 | PXD023325 | JPOST Repository
ProteomicsMembrane-associated effluxosomes coordinate multi-metal resistance in Mycobacterium tuberculosis
Bacterial pathogens must withstand metal-induced stress during infection, yet the mechanisms by which they sense and respond to toxic metal ions remain incompletely understood. Here, we uncover a previously unrecognized mechanism in Mycobacterium tuberculosis, the causative agent of tuberculosis, wh...
ORGANISM(S): Mycobacterium tuberculosis H37Rv 
2025-12-15 | PXD070650 | Pride
Metal binding Tuberculosis Native ms 
ProteomicsOn-chip AP-MS: Interaction proteomics on a microfluidics platform
Essentially all cellular processes are orchestrated by protein-protein interactions (PPIs). In recent years, affinity purification coupled to mass spectrometry (AP-MS) has been the preferred method to identify cellular PPIs. Here we present a microfluidics-based (AP-MS) workflow to identify PPIs usi...
ORGANISM(S): Homo sapiens (Human) 
2019-04-02 | PXD012800 | Pride
Interaction proteomics Low input Ap-ms 
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