Rare variants in NEK1, encoding a serine/threonine kinase, are established contributors to amyotrophic lateral sclerosis (ALS), but the biochemical effects of ALS-associated NEK1 missense variants on kinase activity have not been directly investigated. This project maps the phosphorylation landscape...
Mitosis is an important process in the cell cycle required for cells to divide. Never in mitosis (NIMA)-like kinases (NEKs) are regulators of mitotic functions in diverse organisms. Plasmodium spp, the causative agent of malaria is a divergent unicellular haploid eukaryote with some unusual features...
We and others have found that the NEK1 kinase interacts with the C21ORF2 protein. Gel filtration indicated there may be other proteins in the complex. We tried to identify such proteins by immunoprecipitating endogenous NEK1 from cells and subjecting the precipitates to mass spectrometry. We also im...
