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UnknownA BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation.
Not available
S-EPMC6939940 | biostudies-literature
ProteomicsStarvation-specific NSPs in Leishmania mexicana parasites by quantitative proteomics
Using a combination of BONCAT technique and iTRAQ 3-plex quantitative proteomics MS, we profiled the de novo protein synthesis in the starving L. mexicana parasites in a temporally resolved manner. This approach uniquely integrates the high specificity of the BONCAT technique for the NSPs with the h...
ORGANISM(S): Trypanosoma brucei 
2019-12-04 | PXD015827 | Pride
Newly synthesised proteins Starvation Boncat Leishmania Itraq 
ProteomicsTracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesised proteins
Metaproteomics enables the description of microbial communities (MC). Microbial adaptation to changing environments is conducted by expressing newly synthesized proteins (nP) that can be difficult to distinguish from background proteins. Focusing on nP would add a new dimension to the metaproteomics...
ORGANISM(S): Escherichia coli (strain K12 / W3110 / ATCC 27325 / DSM 5911) Saccharomyces cerevisiae (Baker's yeast) anaerobic digester metagenome 
2024-10-17 | PXD047252 | Pride
Boncat Anaerobic digestion Metaproteomics Microbiome Mass spectrometry Click chemistry 
UnknownT cell detection of a B-cell tropic virus infection: newly-synthesised versus mature viral proteins as antigen sources for CD4 and CD8 epitope display.
Not available
S-EPMC2788701 | biostudies-literature
ProteomicsCell-specific non-canonical amino acid labelling identifies changes in the hippocampal de novo proteome during memory formation
The formation of spatial long-term memory (LTM) requires the de novo synthesis of distinct sets of proteins; however, a non-biased examination of the de novo proteome in this process is lacking. Here, we generated a novel mouse strain, which enables cell-type-specific labelling of newly synthesised ...
ORGANISM(S): Mus musculus (Mouse) 
2020-05-26 | PXD015820 | Pride
Long-term memory Non-canonical amino acid labelling Azidohomoalanine Azidonorleucine Swath Active place avoidance Protein synthesis Mrna splicing 
ProteomicsActively synthesised proteins in human intervertebral disc
The intervertebral disc (IVD) is a joint in the spine that facilitates daily movement, comprising of the central nucleus pulposus (NP), surrounded by the annulus fibrosus (AF) and sandwiched between two cartilage endplates that function together as a unit. Changes to the IVD occur with aging, most d...
ORGANISM(S): Homo sapiens (Human) 
2021-01-29 | PXD018193 | Pride
Proteomics Dynamic proteome Intervertebral disc Silac Proteomic architecture Aging 
UnknownNewly synthesised oxime and lactone derivatives from Dipterocarpus alatus dipterocarpol as anti-diabetic inhibitors: experimental bioassay-based evidence and theoretical computation-based prediction.
Not available
S-EPMC9043233 | biostudies-literature
ProteomicsDecreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling
Tau is a scaffolding protein which serves multiple cellular functions that are perturbed in neurodegenerative diseases, including Alzheimer’s disease (AD) and frontotemporal dementia (FTD). We have recently shown that amyloid-, the second hallmark of AD, induces de novo protein synthesis of tau. Imp...
ORGANISM(S): Mus musculus (Mouse) 
2019-05-17 | PXD013341 | Pride
Tauopathy Non-canonical amino acid labelling Neurodegenerative disease. Protein synthesis Ribosomal proteins 
ProteomicsBONCAT (nascent) proteomes of starved Pseudomonas aeruginosa
We have characterized the responses of the opportunistic pathogen Pseudomonas aeruginosa to prolonged starvation for carbon or nitrogen sources, and to transitions between these states. We find that most cells survive both types of starvation for more than a week and maintain low but robustly detect...
ORGANISM(S): Pseudomonas aeruginosa (strain UCBPP-PA14) Bacteria 
2026-03-04 | PXD069190 | Pride
Nitrogen starvation Microbial dormancy Boncat Label-free quantification Pseudomonas aeruginosa Carbon starvation 
ProteomicsBONCAT in Arabidopsis cell culture LC-MS
Determining which proteins are actively synthesised at a given point in time and being able to extract them for analysis is important to understanding plant responses to environmental or developmental stimuli, but there are few verified experimental tools to do this directly or quantitatively. In-vi...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-06-23 | PXD023170 | Pride
Boncat Arabidopsis Lc-ms Cell culture Noncanoical amino acids 
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