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ProteomicsNHS esters are non-innocent protein acylating reagents
N-Hydroxysuccinimide (NHS)-ester derivatives represent one of the most widely used reagents in biological chemistry and chemical biology. Their perceived utility as acylating agents –intermediates that are sufficiently stable to be isolated (even in commercial form) yet active enough to allow direct...
ORGANISM(S): Escherichia coli 
2025-04-27 | PXD056955 | Pride
Ring-opening Nhs esters N-succinamide derivatives 
ProteomicsNHS esters are non-innocent protein acylating reagents
N-Hydroxysuccinimide (NHS)-ester derivatives represent one of the most widely used reagents in biological chemistry and chemical biology. Their perceived utility as acylating agents –intermediates that are sufficiently stable to be isolated (even in commercial form) yet active enough to allow direct...
ORGANISM(S): Escherichia coli Bacteria 
2025-04-27 | PXD056949 | Pride
Ring-opening reaction N-hydroxysuccinimide (nhs)-ester derivatives N-succinamide derivatives 
UnknownSolution versus gas-phase modification of peptide cations with NHS-ester reagents.
Not available
S-EPMC3265610 | biostudies-literature
UnknownAddressing NHS Chemistry: Efficient Quenching of Excess TMT Reagent and Reversing TMT Overlabeling in Proteomic Samples by Methylamine.
Not available
S-EPMC12004380 | biostudies-literature
ProteomicsNHS Carbamate Cross-Linkers Provide Complementary XL-MS Restraints via Preferential N-Terminal Targeting
Cross-linking mass spectrometry (XL-MS) has become a valuable tool for investigating the structural morphology and plasticity of proteins. Traditional cross-linkers contain two N-hydroxy succinimide (NHS) esters that mainly react with lysine residues. In this work, we optimized the in-solution react...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2026-03-30 | PXD069252 | Pride
Crosslinking ms Cross-linking ms Dsso carbamate Nhs carbamate cross-linkers N-terminal reactivity Xl-ms Cross-linking mass spectrometry Ms-cleavable cross-linkers 
ProteomicsIdentification of Lysine-Cysteine Crosslinking Sites in Homobifunctional NHS-Ester Crosslinkers
NHS esters are commonly used reactive groups in crosslinking chemistry. We first demonstrated that NHS esters can react with the side-chain thiol group of cysteine and observed lysine-cysteine (K-C) crosslinks when using homobifunctional crosslinkers. However, in conventional crosslinking workflows,...
ORGANISM(S): Homo Sapiens Bos Taurus 
2026-03-19 | PXD075905 |
ProteomicsUVPD of peptides modified with N-terminal UV-absorbing chromophores
Ultraviolet photodissociation (UVPD) has emerged as a powerful alternative to conventional collision-induced dissociation (CID) for peptide and protein sequencing in mass spectrometry–based proteomics. However, UVPD efficiency depends on the presence of UV-absorbing chromophores within analytes.The ...
ORGANISM(S): Homo sapiens (Human) 
2026-06-08 | PXD075227 | Pride
Chromophores Lc-ms Proteomics Nhs chemistry Uvpd 
UnknownGas-phase intramolecular protein crosslinking via ion/ion reactions: ubiquitin and a homobifunctional sulfo-NHS ester.
Not available
S-EPMC3644013 | biostudies-literature
UnknownEnhanced Reactivity in Nucleophilic Acyl Substitution Ion/Ion Reactions Using Triazole-Ester Reagents.
Not available
S-EPMC5483193 | biostudies-literature
UnknownCrossing the Solubility Rubicon: 15-Crown-5 Facilitates the Preparation of Water-Soluble Sulfo-NHS Esters in Organic Solvents.
Not available
S-EPMC10797585 | biostudies-literature
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