Sort   by:  
 Page size 
ProteomicsNHS esters are non-innocent protein acylating reagents
N-Hydroxysuccinimide (NHS)-ester derivatives represent one of the most widely used reagents in biological chemistry and chemical biology. Their perceived utility as acylating agents –intermediates that are sufficiently stable to be isolated (even in commercial form) yet active enough to allow direct...
ORGANISM(S): Escherichia coli 
2025-04-27 | PXD056955 | Pride
Ring-opening Nhs esters N-succinamide derivatives 
ProteomicsNHS esters are non-innocent protein acylating reagents
N-Hydroxysuccinimide (NHS)-ester derivatives represent one of the most widely used reagents in biological chemistry and chemical biology. Their perceived utility as acylating agents –intermediates that are sufficiently stable to be isolated (even in commercial form) yet active enough to allow direct...
ORGANISM(S): Escherichia coli Bacteria 
2025-04-27 | PXD056949 | Pride
Ring-opening reaction N-hydroxysuccinimide (nhs)-ester derivatives N-succinamide derivatives 
ProteomicsNHS Carbamate Cross-Linkers Provide Complementary XL-MS Restraints via Preferential N-Terminal Targeting
Cross-linking mass spectrometry (XL-MS) has become a valuable tool for investigating the structural morphology and plasticity of proteins. Traditional cross-linkers contain two N-hydroxy succinimide (NHS) esters that mainly react with lysine residues. In this work, we optimized the in-solution react...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2026-03-30 | PXD069252 | Pride
Crosslinking ms Cross-linking ms Dsso carbamate Nhs carbamate cross-linkers N-terminal reactivity Xl-ms Cross-linking mass spectrometry Ms-cleavable cross-linkers 
UnknownNHS-Esters As Versatile Reactivity-Based Probes for Mapping Proteome-Wide Ligandable Hotspots.
Not available
S-EPMC7771572 | biostudies-literature
UnknownCrossing the Solubility Rubicon: 15-Crown-5 Facilitates the Preparation of Water-Soluble Sulfo-NHS Esters in Organic Solvents.
Not available
S-EPMC10797585 | biostudies-literature
ProteomicsIdentification of Lysine-Cysteine Crosslinking Sites in Homobifunctional NHS-Ester Crosslinkers
NHS esters are commonly used reactive groups in crosslinking chemistry. We first demonstrated that NHS esters can react with the side-chain thiol group of cysteine and observed lysine-cysteine (K-C) crosslinks when using homobifunctional crosslinkers. However, in conventional crosslinking workflows,...
ORGANISM(S): Homo Sapiens Bos Taurus 
2026-03-19 | PXD075905 |
UnknownFacile spectrophotometric assay of molar equivalents of N-hydroxysuccinimide esters of monomethoxyl poly-(ethylene glycol) derivatives.
Not available
S-EPMC3542108 | biostudies-literature
UnknownSite-Specific Protein Labeling with N-Hydroxysuccinimide-Esters and the Analysis of Ubiquitin Ligase Mechanisms.
Not available
S-EPMC6093203 | biostudies-literature
ProteomicsOptimized NHS-amine labeling for multiplexed phosphoproteome study.
In this project, we report the detrimental effect of hydroxylamine, a quenching reagent for NHS-amine reaction, on phosphopeptides. The degree of phosphopeptide enrichment was greatly improved by a desalting method compared with a vacuum-drying method right after quenching reaction. We demonstrate t...
ORGANISM(S): Homo sapiens (Human) 
2018-02-27 | PXD008082 | Pride
Phosphoproteome Β-elimination Hydroxylamine Phosphorylation Tandem mass tag N-hydroxysuccinamide-esters Isobaric tag 
UnknownGas phase reactivity of carboxylates with N-hydroxysuccinimide esters.
Not available
S-EPMC4654944 | biostudies-literature
Sort   by:  
 Page size