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ProteomicsNative protein mapping and visualization of protein interactions in human plasma high-density lipoprotein by 2-DE and LC-MS/MS
A human plasma sample was subjected to non-denaturing micro 2-DE and a gel area (5 mm X 18 mm) which includes high-density lipoprotein (HDL) was cut into 1 mm X 1 mm squares, then the proteins in the 90 gel pieces were analyzed by in-gel trypsin digestion and nano-LC-MS/MS. Grid-cutting of the gel ...
ORGANISM(S): Homo sapiens (Human) 
2014-04-07 | PXD000852 | Pride
High-density lipoproteins Non-denaturing 2-de Nano-lc-ms/ms Lc-mse Plasma Human Hdl 
GenomicsRemodeling of the enhancer landscape during macrophage activation is coupled to enhancer transcription
Recent studies suggest a hierarchical model in which lineage-determining factors act in a collaborative manner to select and prime cell-specific enhancers, thereby enabling signal-dependent transcription factors to bind and function in a cell type-specific manner. Consistent with this model, TLR4 si...
ORGANISM(S): Mus musculus 
2013-08-09 | E-GEOD-48759 | biostudies-arrayexpress
TranscriptomicsMessenger RNA is a functional component of a chromatin insulator complex
Chromatin insulators are DNA-protein complexes situated throughout the genome capable of demarcating independent transcriptional domains. Previous studies point to an important role for RNA in gypsy chromatin insulator function in Drosophila; however, the identity of these putative insulator-associa...
ORGANISM(S): Drosophila melanogaster 
2013-08-06 | E-GEOD-29991 | biostudies-arrayexpress
UnknownPurification and identification of a transcription factor, USF-2, binding to E-box element in the promoter of human telomerase reverse transcriptase (hTERT).
Not available
S-EPMC2851252 | biostudies-literature
GenomicsTranscriptional Architecture and Chromatin Landscape of the Core Circadian Clock in Mammals [ChIP-seq]
The mammalian circadian clock involves a transcriptional feedback loop in which CLOCK and BMAL1 activate the Period and Cryptochrome genes, which then feedback and repress their own transcription. We have interrogated the transcriptional architecture of the circadian transcriptional regulatory loop ...
ORGANISM(S): Mus musculus 
2012-08-31 | E-GEOD-39977 | biostudies-arrayexpress
UnknownEnrichment and Analysis of Intact Phosphoproteins in Arabidopsis Seedlings.
Not available
S-EPMC4497735 | biostudies-literature
UnknownHigh-density lipoprotein (HDL) particle size and concentration changes in septic shock patients.
Not available
S-EPMC6565796 | biostudies-literature
ProteomicsSelf-packed size-exclusion columns enable versatile high-throughput native, top-down, and ion-mobility-mass spectrometry studies on proteins and complexes.
Native MS (nMS) is a key structural biology technique that makes it possible to study intact proteins and their interactions. Unfortunately, non-volatile salts are incompatiblewith nMS, which demands a laborious desalting procedure. Non-denaturing size exclusion chromatography (SEC) allows both rapi...
ORGANISM(S): Bos taurus (Bovine) Candida albicans (Yeast) Homo sapiens (Human) Escherichia coli Gallus gallus (Chicken) Equus caballus (Horse) 
2025-06-29 | PXD061452 | Pride
Ligand screening Top-down proteomics Native mass spectrometry Size-exclusion chromatography Protac Ion mobility 
ProteomicsHuman plasma peptidomics - Identification and quantification of plasma free salusin-β, an endogenous parasympathomimetic peptide
We have improved our ultrahigh-yield method, which we named “differential solubilization" (J Proteome Res 9, 2010, 1694-1705), to extract and analyse low-molecular-weight plasma peptides, and initiated a large-scale sequencing of circulating native peptides at concentrations as low as the picomolar ...
ORGANISM(S): Homo sapiens (Human) 
2018-10-26 | PXD003533 | Pride
Plasma Native peptide Peptidome 
TranscriptomicsTranscription profiling of 5 to 7 days old Arabidopsis thaliana seedlings of wild type (Col-0) and the cytokinin deficient transgenic line 35S:CKX1
5 to 7 days old Arabidopsis thaliana seedlings of wildtype (Col-0) and the cytokinin deficient transgenic line 35S:CKX1 (Werner et al. Plant Cell 15, 2532) at growth stage 1.00 (Boyes scale) grown in liquid medium were induced witn 5 µM 6-Benzylaminopurine for different periods of time in order to f...
ORGANISM(S): Arabidopsis thaliana 
2005-07-31 | E-MEXP-344 | biostudies-arrayexpress
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