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MetabolomicsLC-MS/MS Quantification of Nuclear α-KG in NSCLC
This study contains targeted LC-MS/MS quantification of α-ketoglutarate (α-KG) in NSCLC cells using 13C-labeled α-KG internal standard. Nuclear α-KG levels were measured in A549, A549/DTX, PC9, and PC9/OR cells. In addition, nuclear α-KG was quantified in GLUD1-overexpressing A549 cells and GLUD1-kn...
2026-07-21 | MTBLS15115 | MetaboLights
TranscriptomicsBiochemical fractionation of HEK293 nuclei and RNA-seq of chromatin-associated and soluble-nuclear RNA
Biochemical fractionation of HEK293 nuclei and RNA-seq of chromatin-associated and soluble-nuclear RNA. Nuclei from three biological replicates were isolated by detergent lysis, fractionated, then three chromatin and three soluble RNA samples were converted to cDNA using Illumina TruSeq stranded pro...
ORGANISM(S): Homo sapiens 
2015-03-04 | E-GEOD-66478 | biostudies-arrayexpress
TranscriptomicsEfficient cellular fractionation of RNA
The aim of this experiment was to develop new methods to extract pure fractions of nuclear and cytoplasmic RNA. We compared the results of nuclear and cytoplasmic RNA-sequencing to results of the total and polyA+ RNA-sequencing. Cytoplasmic, nuclear, total and polyA+ RNA was extracted from two human...
ORGANISM(S): Homo sapiens 
2013-10-22 | E-MTAB-1898 | biostudies-arrayexpress
TranscriptomicsCytoplasmic Assembly and Selective Nuclear Import of Arabidopsis ARGONAUTE4/siRNA Complexes
Here we profiled small RNAs from whole cell, cytoplasmic and nuclear extracts from three-week-old Arabidopsis seedlings. We unexpectedly found that nuclear functional hc-siRNAs are predominantly present in the cytoplasm. Samples from Arabidopsis thaliana whole cell, cytoplasmic and nuclear extract...
ORGANISM(S): Arabidopsis thaliana 
2012-07-12 | E-GEOD-36500 | biostudies-arrayexpress
GenomicsCytoplasmic and nuclear fractionation from HEK293T cells
Cytoplasmic and nuclear fractionation from HEK293T cells
PRJNA800803 | ENA
GenomicsPhosphorylation controls mRNA retention via nuclear speckle cohesion - cellular fractionation data
Phosphorylation controls mRNA retention via nuclear speckle cohesion - cellular fractionation data
PRJNA1005639 | ENA
ProteomicsHEK293T cell nuclear & cytosolic phospho-proteome remodelling upon amino acid withdrawal. Related to Supplementary Table 4 from An et al (2020)
HEK293T Phospho proteome cytosolic vs nuclear fractionation upon short (3h) amino acids withdrawal.
ORGANISM(S): Homo sapiens (Human) 
2020-04-30 | PXD017855 | Pride
Human Lc-msms Cytosolic nuclear fractionation Phospho 
ProteomicsHEK293T cell nuclear & cytosolic proteome remodelling upon amino acid withdrawal. Related to Supplementary Table 4 from An et al (2020)
HEK293T proteome cytosolic vs nuclear fractionation upon short (3h) amino acids withdrawal.
ORGANISM(S): Homo sapiens (Human) 
2020-04-30 | PXD017856 | Pride
Human Lc-msms Cytosolic nuclear fractionation 
TranscriptomicsRNA-sequencing in SH-SY5Y neuroblastoma cell line with TDP-43 KD and nuclear and cytoplasmic fractionation as well and treatment with the nonsense-mediated decay inhibition using SMG1-11j inhibitor
TDP-43 knockdown produces aberrant NMD-sensitive cryptic exons. To discover where TDP-43 cryptic exons were localized and which were sensitive to NMD-inhibition, we performed cellular fractionation as well and NMD-inhibition with TDP-43 KD
ORGANISM(S): Homo sapiens 
2026-07-04 | E-MTAB-15653 | biostudies-arrayexpress
TranscriptomicsGenomewide demarcation of RNA PolII transcription units by physical fractionation of chromatin- ORF enrichment
Epigenetic modifications of chromatin serve an important role in regulating the expression and accessibility of genomic DNA. We report here a genomewide approach for fractionating yeast chromatin into two functionally distinct parts, one containing RNA polymerase II transcribed sequences, and the ot...
ORGANISM(S): Saccharomyces cerevisiae 
2006-08-30 | E-GEOD-5649 | biostudies-arrayexpress
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