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ProteomicsEmiliania Huxleyi exposed to 4 treatments of HHQ
Emiliania huxleyi: Cellular cascades induced by bacterial algicides Interactions between phytoplankton and bacteria play a central role in mediating oceanic biogeochemical cycling and microbial trophic structure in the ocean. The intricate relationships between these two domains of life are mediated...
ORGANISM(S): Emiliania huxleyi 
2020-01-07 | PXD011560 | Pride
Quorum sensing bacteria and phytoplankton 
ProteomicsBering Strait surface water and Chukchi Sea bottom water microbiome metaproteomics
Analysis of bacterial fraction collected on GF/F filters post pre-filtration on 1um filter. 15L were filtered from Bering Strait (BSt) surface water and Chukchi Sea (station 2) bottom waters.
ORGANISM(S): marine metagenome 
2019-01-25 | PXD006472 | Pride
Bering strait surface water and chukchi sea bottom water microbiome metaproteomics 
Proteomicsmicrobiome of methane hydrate sediments: Hydrate Ridge
Gas hydrates, also known as clathrates, are cages of ice-like water crystals encasing gas molecules such as methane (CH4). Despite the global importance of gas hydrates, their microbiomes remain mysterious. Microbial cells are physically associated with hydrates, and the taxonomy of these hydrate-...
ORGANISM(S): sediment metagenome 
2020-01-22 | PXD012479 | Pride
High-pressure Cold Hydrate-associated microbiome Bacteria Atribacteria Metaproteomics 
ProteomicsProteomic analysis of coral symbionts exposed to thermal stress: Acropora millipora and Symbiodinium adriaticum
A thermal stress experiment on Heron Island (Great Barrier Reef) which involved a slow ramp in temperature over one week and then sampling after 4 days at 32 degrees was completed. Control samples were maintained at 27C. The idea of the experiment was to study bleaching from a single cell perspectiv...
ORGANISM(S): Symbiodinium sp. ex Acropora millepora 
2019-01-24 | PXD011668 | Pride
Symbionts Phytoplankton climate change Coral Acropora millipora Thermal stress 
ProteomicsCompetition between microbes during anaerobic oxidation of methane: Bioreactor metaproteomics
In this study, we investigated Mn3+-cycling microbial populations enriched from Lake Matano, Indonesia using metagenomics and metaproteomics. Lake Matano contains an active Mn cycle that links the oxic-anoxic interface with anoxic deep waters that are enriched in iron and manganese, and depleted in ...
ORGANISM(S): sediment metagenome 
2020-06-30 | PXD011642 | Pride
Dechloromonas Bioreactor Lake sediment 
ProteomicsGeoduck (Panopea generosa) gonad LC-MS/MS DIA
Geoduck clams (Panopea generosa) were collected from Puget Sound, WA in November, 2014. Male and female geoduck gonads were sampled at three reproductive stages over the course of three months: early, middle, and late. Early stage indicates that the gonad cells are just beginning to differentiate an...
ORGANISM(S): Panopea generosa 
2017-01-02 | PXD004921 | Pride
Gonad Dia Geoduck Panopea Reproduction 
ProteomicsGeoduck (Panopea generosa) gonad LC-MS/MS
Geoduck clams (Panopea generosa) were collected from Puget Sound, WA in November, 2014. Male and female geoduck gonads were sampled at three reproductive stages over the course of three months: early, middle, and late. Early stage indicates that the gonad cells are just beginning to differentiate an...
ORGANISM(S): Panopea generosa 
2018-12-04 | PXD003127 | Pride
Clam Geoduck Geoduck Panopea Reproduction 
ProteomicsProteomics of Colwellia psychrerythraea grown at multiple subzero temperatures
The mechanisms that allow psychrophilic bacteria to remain metabolically active at subzero temperatures result from form and function of their proteins. We present first proteomic evidence of physiological changes of the marine psychrophile Colwellia psychrerythraea 34H (Cp34H) after exposure to sub...
ORGANISM(S): Colwellia psychrerythraea 34H 
2019-02-15 | PXD006274 | Pride
Alteromonadaceae/metabolism Dna repair Proteomics Adaptation physiological/genetics Nitrogen/metabolism Bacterial proteins/genetics Movement Iron/metabolism Colwellia pschrerythraea Bacterial proteins/metabolism Alteromonadaceae/genetics Cold temperature 
ProteomicsCritical decisions in metaproteomics: achieving high confidence protein annotations in a sea of unknowns.
Environmental meta-omics is rapidly expanding as sequencing capabilities improve, computing technologies become more accessible, and associated costs are reduced. The in situ snapshots of marine microbial life afforded by these data provide a growing knowledge of the functional roles of communities ...
ORGANISM(S): marine metagenome 
2019-01-25 | PXD006688 | Pride
Metaproteomics  ocean metaproteomics 
ProteomicsFunctional Response of Polar Bacterial Communities under Variable Organic Inputs Revealed with Metaproteomic, 16S rRNA and Organic Compositional Analysis
Organic matter recycling in marine systems is largely driven by microbial processes, particularly in the Arctic where primary production and inputs can be temporally offset from upper trophic level consumption. This study followed bacterial dynamics in the chlorophyll maximum of the Bering Strait an...
ORGANISM(S): marine metagenome 
2019-02-18 | PXD008780 | Pride
Metaproteomics Metagenomics Bacteria Arctic Organic matter 
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