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TranscriptomicsExpression data from human sarcoma patient samples treated with either vehicle control or Nutlin-3a
This study has examined the molecular mechanisms underlying sensitivity of sarcomas to Nutlin-3a, a non-genotoxic activator of the p53 pathway. Human patient material was collected immediately following surgical resection, dissected into small pieces and ex planted onto gelatin sponges immersed in m...
ORGANISM(S): Homo sapiens 
2014-03-10 | E-GEOD-48296 | biostudies-arrayexpress
TranscriptomicsGlobal gene expression analysis in macrophages treated with Nutlin-3
The goal of the study is to identify p53 target genes specific to macrophages using the p53 stabilizer, Nutlin-3. Macrophages were differentiated x-vivo from monocytes obtained from healthy volunteers either left untreated or treated for 2 hours with 100 ng/mL of lipopolysaccharide (LPS), 0.1%v/v of...
ORGANISM(S): Homo sapiens 
2014-05-21 | E-GEOD-43596 | biostudies-arrayexpress
TranscriptomicsHCT116 cells isogenic for p53 expression upon treatment with actinomycin D and nutlin-3
HCT116 wild type cells express p53. The isogenic HCT116 p53KO cells have the p53 gene knocked out. Cells were treated for 16 hours. Dosages for actinomycin D treatment was 1 nM, 10 nM, and 100 nM. Dosage for nutlin-3 was 4 uM. Keywords: dose response Measuring the gene expression profile of the is...
ORGANISM(S): Homo sapiens 
2010-06-24 | E-GEOD-12459 | biostudies-arrayexpress
TranscriptomicsGlobal analysis of p53-regulated transcription reveals its direct targets and unexpected regulatory mechanisms (GRO-Seq)
GRO-seq of HCT116 cells treated with Nutlin to activate p53 HCT116 cells were treated with Nutlin or DMSO for 1 hour
ORGANISM(S): Homo sapiens 
2014-05-15 | E-GEOD-53964 | biostudies-arrayexpress
TranscriptomicsDiverse stresses dramatically alter genome-wide p53 binding and transactivation landscape in human cancer cells (Affymetrix)
The effects of diverse stresses on promoter selectivity and transcription regulation by the tumor suppressor p53 are poorly understood. We have taken a comprehensive approach to characterizing the human p53 network that includes p53 levels, binding, expression and chromatin changes under diverse str...
ORGANISM(S): Homo sapiens 
2013-07-01 | E-GEOD-46493 | biostudies-arrayexpress
ProteomicsInterruption of p53-MDM2 Interaction by Nutlin-3a in Human Lymphoma Cell Models Initiates a Cell-Dependent Global Effect on Transcriptome and Proteome Level
In most lymphomas, p53 signaling pathway is inactivated by various mechanisms independent to p53 gene mutations or deletions. In many cases, p53 function is largely regulated by alterations in the protein abundance levels by the action of E3 ubiquitin-protein ligase MDM2, targeting p53 to proteasome...
ORGANISM(S): Homo sapiens (Human) 
2023-07-30 | PXD009290 | Pride
Proteomics Lymphoma Mdm2 Apoptosis Transcriptomics Systems biology Nutlin 3a P53 
TranscriptomicsMicroRNA profiling by array of human multiple myloma p53 wild type cells treated with nutlin 3A
The miRNA profiles were analyzed for nutlin 3A treated and untreated multiple myloma p53 wild type cells
ORGANISM(S): Homo sapiens 
2010-07-22 | E-TABM-1038 | biostudies-arrayexpress
ProteomicsNutlin-3a-induced changes in the proteome of parental and RFX7 knock-out U2OS cells
Mass spectrometry-based whole proteome analysis of parental and RFX7 knock-out U2OS cells treated with 10 µM Nutlin-3a or DMSO solvent control. Ten biological replicates were used.
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD024869 | Pride
Proteomics Rfx7 Nutlin Tumor 
GenomicsATAC-seq from Nutlin-3a-treated MCF-7 cells
ATAC-seq from Nutlin-3a-treated MCF-7 cells
PRJNA1051614 | ENA
ProteomicsTranscriptomic and proteomic study of cancer cell lines exposed to actinomycin D and nutlin-3a reveals numerous, novel candidates for p53-regulated genes
Transcriptomic analyses revealed hundreds of p53-regulated genes, however these studies used a limited number of cell lines and p53-activating agents. Therefore, we searched for candidate p53-target genes by employing stress factors and cell lines never used in high-throughput search for p53-regulat...
ORGANISM(S): Homo sapiens (Human) 
2026-05-31 | PXD036860 | Pride
Transcriptome Secretome P53  innate immunity  wnt signaling 
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