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ProteomicsHuman testis off-line LC-MS/MS
In order to identify more MPs and get deeper proteomic data of testis. We applied high-pH reverse phase (RP) HPLC to fractionate complex samples.On the other hand, to enhance protein coverage, multi-protease strategies were used for 10% SDS-PAGE short separation samples.
ORGANISM(S): Homo sapiens (Human) 
2018-10-04 | PXD009737 | Pride
Off-line rp-hplc  multi-proteases  testis  missing proteins 
ProteomicsHuman testis off-line LC-MS/MS
In order to identify more MPs and get deeper proteomic data of testis. We applied high-pH reverse phase (RP) HPLC to fractionate complex samples.On the other hand, to enhance protein coverage, multi-protease strategies were used for 10% SDS-PAGE short separation samples.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-11-19 | MSV000086491 | MassIVE
ProteomicsThermal proteome profiling of Arabidopsis thaliana
Six biological replicates of Arabidopsis thaliana were subjected to protein extraction, ten-temperature gradient precipitation, TMT labeling, off-line alkaline fractionation, and LC-MS/MS. The resulting data was used downstream for untargeted modeling of protein unfolding and melting temperature est...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-11-23 | PXD011200 | Pride
Lc-ms Arabidopsis Tpp 
UnknownIntegrative network analysis of signaling in human CD34(+) hematopoietic progenitor cells by global phosphoproteomic profiling using TiO2 enrichment combined with 2D LC-MS/MS and pathway mapping.
Not available
S-EPMC3883140 | biostudies-literature
UnknownThe antioxidant potential of peptides obtained from the spotted babylon snail (Babylonia areolata) in treating human colon adenocarcinoma (Caco-2) cells.
Not available
S-EPMC9055304 | biostudies-literature
UnknownA novel peptide derived from Zingiber cassumunar rhizomes exhibits anticancer activity against the colon adenocarcinoma cells (Caco-2) via the induction of intrinsic apoptosis signaling.
Not available
S-EPMC11175412 | biostudies-literature
ProteomicsFollicular cells Top-down proteomic
In mammals, the capacity of the female germ cell, the oocyte, to develop into embryo is acquired throughout meiotic maturation. Immature oocyte cannot be fertilized while mature oocyte is apt to accept spermatozoa and to develop an embryo. In a follicle, the oocyte is surrounded by mural granulosa c...
ORGANISM(S): Bos taurus (Bovine) 
2017-04-10 | PXD004892 | Pride
Oocytes Μlc-ms/ms Cumulus cells Top-down proteomic Granulosa cells 
ProteomicsImpact of graded calorie restriction on mouse liver protein expression
Calorie restriction (CR) extends lifespan by modulating the mechanisms involved in aging. We quantified the hepatic proteome of male C57BL/6 mice exposed to graded levels of CR (0% to 40% CR) for three months, and evaluated which signaling pathways were most affected.
ORGANISM(S): Mus musculus (Mouse) 
2023-10-24 | PXD033436 | Pride
Mouse Proteome Calorie restriction Liver 
ProteomicsSimPLIT: Simplified sample preparation for large-scale isobaric tagging proteomics
Large scale proteomic profiling of cell lines can yield valuable insights into the molecular signatures attributed to variable genotypes or induced perturbations. Specifically, the ability to perform deep and rapid proteome analysis of pharmacologically modulated cells could generate drug-protein as...
ORGANISM(S): Homo sapiens (Human) 
2022-08-03 | PXD031510 | Pride
Tmtpro Imids Celmods Targeted protein degradation Cancer cell lines Isobaric labeling 
ProteomicsSimPLIT: Simplified sample preparation for large-scale isobaric tagging proteomics
Large scale proteomic profiling of cell lines can yield valuable insights into the molecular signatures attributed to variable genotypes or induced perturbations. Specifically, the ability to perform deep and rapid proteome analysis of pharmacologically modulated cells could generate drug-protein as...
ORGANISM(S): Homo sapiens (Human) 
2022-08-03 | PXD034022 | Pride
Imids Tmtpro Celmods Targeted protein degradation Cancer cell lines Isobaric labeling 
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