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ProteomicsAnti-Flag AP-MS of cytoplasmic 3×Flag-importin α1 complexes in HeLa S3 cells
To identify binding partners of importin α1 in the cytoplasm, co-immunoprecipitation using an anti-Flag antibody in the cytoplasmic fraction of 3×Flag–importin α1-inducible HeLaS3 cells.
ORGANISM(S): Homo sapiens (Human) 
2026-04-22 | PXD076745 | Pride
Nuclear localization signal Helas3 Importin Nls 
TranscriptomicsMethylation status in AG- and PG-derived cells and sperm
Genomic imprinting describes the expression of a subset of mammalian genes from one parental chromosome. The parent-of-origin specific expression of imprinted genes relies on DNA methylation of CpG-dinucleotides at differentially methylated regions (DMRs) during gametogenesis. We identified the pate...
ORGANISM(S): Mus musculus 
2010-05-04 | E-GEOD-18126 | biostudies-arrayexpress
TranscriptomicsMethylation status in human sperm
Genomic imprinting describes the expression of a subset of mammalian genes from one parental chromosome. The parent-of-origin specific expression of imprinted genes relies on DNA methylation of CpG-dinucleotides at differentially methylated regions (DMRs) during gametogenesis. We identified the pate...
ORGANISM(S): Homo sapiens 
2010-05-05 | E-GEOD-18178 | biostudies-arrayexpress
UnknownAlternative mRNA Splicing in Three Venom Families Underlying a Possible Production of Divergent Venom Proteins of the Habu Snake, Protobothrops flavoviridis.
Snake venoms are complex mixtures of toxic proteins encoded by various gene families that function synergistically to incapacitate prey. A huge repertoire of snake venom genes and proteins have been reported, and alternative splicing is suggested to be involved in the production of divergent gene tr...
S-EPMC6832531 | biostudies-literature
ProteomicsNuclear and total proteins in THP-1 cell differentiation
THP-1 monocyte-like suspension cells can be differentiated into macrophage-like adherent cells by TPA (PMA) treatment. Proteomic analysis was carried out on (i) normal THP-1 cells, (ii) THP-1 cells in which KPNA1 (importin-alpha5) was knocked down or not by siRNA, and (iii) THP-1 TetOn-KPNA2 (import...
ORGANISM(S): Homo Sapiens (human) 
2025-02-15 | PXD049401 | JPOST Repository
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