Sort   by:  
 Page size 
ProteomicsRefinement of paramagnetic bead–based digestion protocol for automatic sample preparation using an artificial neural network
Despite technological advances in the proteomics field, sample preparation still represents the main bottleneck in mass spectrometry (MS) analysis. Bead-based protein aggregation techniques have recently emerged as an efficient, reproducible, and high-throughput alternative for protein extraction an...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Mus musculus (Mouse) Homo sapiens (Human) Escherichia coli Rattus norvegicus (Rat) 
2024-05-31 | PXD048358 | Pride
Bile Magnetic beads Protein aggregation Sp3 protein digestion Artificial neural network 
ProteomicsChemical acetylation of ligands and two-step digestion protocol for reducing co-digestion in affinity purification-mass spectrometry
We present an effective, fast and user-friendly method to reduce co-digestion of bead-bound ligands, such as antibodies or streptavidin, in affinity purification-mass spectrometry experiments. A short pre-incubation of beads with Sulfo-NHS-Acetate leads to chemical acetylation of lysine residues, ma...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2023-10-23 | PXD043709 | Pride
Chemical acetylation Immunopurification Streptavidin Ligand Affinity purification Sulfo-nhs-acetate Nanobody Gfp 
ProteomicsLC/MS Analysis of Myc-Affinity Captured, on-Beads Crosslinked, and on-Beads Digested S. cerevisiae SCC2 subunit of cohesin loading factor
Myc-Affinity Capture and on-Beads Crosslinking For each Saccharomyces cerevisiae strain (C-terminally myc-tagged Scc2 subunit of the cohesin loading factor complex or wild-type BY4741), 2x 3 L YPD medium (add 300 ml 20% Glucose to each 3 L of YPD before using) were separately inoculated in a 5L flas...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2020-10-20 | MSV000086339 | MassIVE
ProteomicsInfluence of bead chemistry and protein-to-bead ratio on the efficiency of solid-phase proteolysis
Optimization of an SP3 digestion workflow on human plasma samples. The performance of the SP3 workflow using beads with different surface chemistries (SpeedBeads and MagReSyn beads) was evaluated.
ORGANISM(S): Homo sapiens (Human) 
2026-02-24 | PXD065651 | Pride
Sp3 Plasma Digestion 
UnknownQuantitative Assessment of the Effects of Trypsin Digestion Methods on Affinity Purification-Mass Spectrometry-based Protein-Protein Interaction Analysis.
Not available
S-EPMC5656008 | biostudies-literature
ProteomicsFully automated workflow for integrated sample digestion and Evotip loading enabling high-throughput clinical proteomics
Protein identification and quantification is an important tool for biomarker discovery. With the increased sensitivity and speed of modern mass spectrometers, sample-preparation remains a bottleneck for studying large cohorts. To address this issue, we prepared and evaluated a simple and efficient w...
ORGANISM(S): Homo sapiens (Human) 
2024-06-12 | PXD048325 | Pride
Automation Plasma proteomics Dia Biomarkers 
UnknownChemical Acetylation of Ligands and Two-Step Digestion Protocol for Reducing Codigestion in Affinity Purification-Mass Spectrometry.
Not available
S-EPMC10563155 | biostudies-literature
UnknownTrypsin coatings on electrospun and alcohol-dispersed polymer nanofibers for a trypsin digestion column.
Not available
S-EPMC2950708 | biostudies-literature
ProteomicsThiol-cleavable Biotin for Chemical and Enzymatic Biotinylation and its Application to Mitochondrial TurboID Proteomics
Protein biotinylation via chemical or enzymatic reactions is often coupled with streptavidin-based enrichment and on-beads digestion in numerous biological applications. However, the popular on-beads digestion method faces major challenges of streptavidin contamination, the lost information of bioti...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-04-21 | MSV000087256 | MassIVE
Unknown<i>In Vitro</i> Digestion and Fermentation of Cowpea Pod Extracts and Proteins Loaded in Ca(II)-Alginate Hydrogels.
Not available
S-EPMC11475951 | biostudies-literature
Sort   by:  
 Page size