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GenomicsPhysically Isolated OptiPrep (TM) Fractions for exRNA Analysis
Physically Isolated OptiPrep (TM) Fractions for exRNA Analysis
PRJNA510087 | ENA
UnknownIsolation of senescent cells by iodixanol (OptiPrep) density gradient-based separation.
Not available
S-EPMC6869531 | biostudies-literature
UnknownProteomic characterisation of leech microglia extracellular vesicles (EVs): comparison between differential ultracentrifugation and Optiprep™ density gradient isolation.
Not available
S-EPMC6493217 | biostudies-literature
ProteomicsTrichuris secretome - proteome data analysis
We analysed the excretory/secretory (ES) products and the exosomes secreted by the nematode Trichuris muris. Two batches of ES and Optiprep fractions of exosomes were analysed using a 5600+ mass spectrometer
ORGANISM(S): Trichuris muris 
2017-05-23 | PXD006344 | Pride
Exosomes Trichuris muris Proteome Secretome 
TranscriptomicsTranscription profiling of human early and late pubertal females reveals a brief bout of exercise alters gene expression and distinct gene pathways in PBMC
We compared PBMC genomic response to exercise in both early (EG) and late-pubertal girls (LG) Experiment Overall Design: Twenty healthy females (age range 8-17 y/o) participated in this study. A blood sample was taken before the onset of exercise and immediately after 30-min exercise bout. PBMCs wer...
ORGANISM(S): Homo sapiens 
2009-06-26 | E-GEOD-14642 | biostudies-arrayexpress
TranscriptomicsEffects of exercise on miRNA expression in human peripheral blood mononuclear cells (PBMCs)
Exercise leads to a rapid change in the profile of gene expression in circulating PBMCs. We hypothesized that miRNA expression in circulating PBMCs would also be affected by brief exercise. 12 healthy men (19-30 yr old) performed 10 2-min bouts of cycle ergometer exercise interspersed with 1-min res...
ORGANISM(S): Homo sapiens 
2011-09-25 | E-GEOD-28745 | biostudies-arrayexpress
TranscriptomicsPhysically Isolated OptiPrep (TM) Fractions for exRNA Analysis
An integrative analysis of human biofluid data in the exRNA Atlas revealed the existence of distinct extracellular RNA cargo types. In order to detect differences in density between cargo types, cushioned density gradient ultracentrifugation (C-DGUC) of serum and plasma was performed using OptiPrem ...
ORGANISM(S): Homo sapiens 
2018-12-15 | GSE123864 | GEO
ProteomicsA sensitive S-trap-based approach to the analysis of T-cell raft proteome
The development of sensitive technologies for T cell-raft proteomics is highly challenging. We explored an innovative strategy, based on suspension trapping (S-trap) sample preparation. Mouse splenocytes were subjected to negative immunoselection for T cell preparation. Rafts were isolated by a dete...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2020-08-13 | PXD016476 | Pride
T cell Optiprep Raftomics Fasp S-trap Detergent-free 
UnknownAn improvised one-step OptiPrep cushion ultracentrifugation method for outer membrane vesicles isolation of Klebsiella pneumoniae.
Not available
S-EPMC11681735 | biostudies-literature
ProteomicsBreast cancer exosome protein profiling using TMT quantitation and SVM analysis
Exosomes are microvesicles of endocytic origin constitutively released by multiple cell types into the extracellular environment. With evidence that exosomes can be detected in the blood of patients with various onco-malignancies, the development of a platform that uses exosomes as a diagnostic tool...
ORGANISM(S): Homo sapiens (Human) 
2015-10-02 | PXD002053 | Pride
Human Exosomes Tmt quantitation Breact cancer 
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