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ProteomicsFunctional proteomics-based investigation of the cellular response to farnesyl transferase inhibition in lung cancer (subcellular localization and relocalization analysis)
The subcellular localization of proteins can be highly dynamic and regulated by post-translational modifications. Farnesylation is a lipid modification that enables anchoring of proteins to cellular membranes, and thus, spatially resolved signaling and activity. Due to the importance of farnesylatio...
ORGANISM(S): Homo sapiens (Human) 
2025-05-19 | PXD050490 | Pride
Proteomics Tipifarnib Lung cancer Human Farnesylation Subcellular Hirief Cell line Lc-ms/ms 
ProteomicsLabel-free mass spectrometry-based proteomics of lung cancer FFPE scrolls
Formalin-fixed, paraffin-embedded (FFPE) is the most common method for preserving tissue material in the clinic with millions of specimens stored in biobanks around the world. However, FFPE samples present challenges for molecular profiling, including proteomics. Nonetheless, optimized sample prepar...
ORGANISM(S): Homo sapiens (Human) 
2026-05-26 | PXD071948 | Pride
Dia Ffpe Label-free proteomics Tumor Lung cancer Lc-ms/ms 
ProteomicsLabel-free mass spectrometry-based proteomics of lung cancer FFPE glass slides
Formalin-fixed, paraffin-embedded (FFPE) is the most common method for preserving tissue material in the clinic with millions of specimens stored in biobanks around the world. However, FFPE samples present challenges for molecular profiling, including proteomics. Nonetheless, optimized sample prepar...
ORGANISM(S): Homo sapiens (Human) 
2026-05-26 | PXD072014 | Pride
Dia Ffpe Label-free proteomics Tumor Lung cancer Lc-ms/ms 
ProteomicsFunctional proteomics-based investigation of the cellular response to farnesyl transferase inhibition in lung cancer (tipifarnib treatment of lung cancer cells)
The subcellular localization of proteins can be highly dynamic and regulated by post-translational modifications. Farnesylation is a lipid modification that enables anchoring of proteins to cellular membranes, and thus, spatially resolved signaling and activity. Due to the importance of farnesylatio...
ORGANISM(S): Homo sapiens (Human) 
2025-05-13 | PXD050480 | Pride
Proteomics Tipifarnib Lung cancer Human Hirief Cell line Lc-ms/ms 
Proteomics: Functional proteomics-based investigation of the cellular response to farnesyl transferase inhibition in lung cancer (PTP4A1 silencing and IFN treatment of NCI-H1944 cells)
The subcellular localization of proteins can be highly dynamic and regulated by post-translational modifications. Farnesylation is a lipid modification that enables anchoring of proteins to cellular membranes, and thus, spatially resolved signaling and activity. Due to the importance of farnesylatio...
ORGANISM(S): Homo sapiens (Human) 
2025-05-13 | PXD050446 | Pride
Proteomics Lung cancer Human Interferon Ptp4a1 Hirief Cell line Lc-ms/ms 
ProteomicsFunctional proteomics-based investigation of the cellular response to farnesyl transferase inhibition in lung cancer (farnesylated proteins pull-down)
The subcellular localization of proteins can be highly dynamic and regulated by post-translational modifications. Farnesylation is a lipid modification that enables anchoring of proteins to cellular membranes, and thus, spatially resolved signaling and activity. Due to the importance of farnesylatio...
ORGANISM(S): Homo sapiens (Human) 
2025-04-09 | PXD050725 | Pride
Proteomics Lung cancer Human Farnesylation Cell line Lc-ms/ms 
ProteomicsSubCellBarCode: Integrated workflow for robust classification and visualization of spatial proteome
We have developed a mass spectrometry (MS) and bioinformatics-based pipeline to generate a proteome-wide resource of protein subcellular localization across multiple human cancer cell lines (www.subcellbarcode.org). Here, we present a detailed wet-lab protocol spanning from subcellular fractionation...
ORGANISM(S): Homo Sapiens (human) 
2022-09-26 | PXD022533 | JPOST Repository
ProteomicsE.Coli spike in to human cell line MCF-7
Spike in of 3 different amounts of E.Coli to human cell line MCF-7 with TMT10plex quantification.
ORGANISM(S): Homo Sapiens (human) 
2020-03-28 | PXD013277 | JPOST Repository
ProteomicsSubCellBarcode: Scalable proteome-wide mapping of protein localization and relocalization
We have devised a simple and robust LC-MS/MS compatible protocol for subcellular protein localization studies. Here, we combine it with in depth proteome profiling and utilize the resulting dataset to predict the subcellular localization of more than 12000 proteins in 5 human cancer cell lines. The ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-02-17 | MSV000086899 | MassIVE
Proteomics,MultiomicsHiRIEF deep proteomics of A431 human vulvar cancer cell line and N2A mouse neuroblastoma cell line
Here we present high resolution data from two cancer cell lines. A human cell line, A431 was treated with a drug at different time points and then fractionated into its different subcellular compartments (nuclear, organellar, soluble,whole cell). Protein extracts were trypsinized, peptides separated...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
2016-07-20 | MSV000079945 | MassIVE
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