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ProteomicsRemoval of NHS-labelling by-products in proteomic samples
N-Hydroxysuccinimide chemistry is used extensively across proteomics sample preparation. One of the most popular applications is its use in isobaric reagent based quantitation; specifically both the iTRAQ and TMT approaches which rely on complete labelling of primary amines. However, serines, tyrosi...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2025-03-14 | PXD056723 | Pride
Phospho-peptides Tmt N-hydroxy succinimide Methylamine Nhs-ester Hydroxylamine 
ProteomicsRemoval of NHS-labelling by-products in proteomic samples
N-Hydroxysuccinimide (NHS) chemistry is used extensively across proteomics sample preparation. One of the most popular applications is its use in isobaric reagent based quantitation; specifically both the iTRAQ and TMT approaches. The labels are introduced onto primary Serines, tyrosines, and threon...
ORGANISM(S): Homo sapiens (Human) 
2025-03-14 | PXD054730 | Pride
Nhs Methylamine Hydroxylamine N-hydroxysuccinimide O-acylation 
TranscriptomicsSaccharomyces cerevisiae sorted and untreated
Gene expression of a vital, stained and sorted subpopulation of Saccharomyces cerevisiae with high affinity to glucose, harvested at a dilution rate of D=0.160 h-1, and of cells from the whole population without further treatment grown at the same dilution rate were analysed. The isolation of RNA wa...
ORGANISM(S): Saccharomyces cerevisiae 
2007-10-01 | E-GEOD-8247 | biostudies-arrayexpress
TranscriptomicsComparison between Saccharomyces cerevisiae HS 10, HS 34 and HS 12, HS 37
Gene expression of different flocculent (HS 10 and HS 34) and powdery (HS 12 and HS 37) yeast strains compared to each other during exponential and stationary growth phase was analysed. The isolation of RNA was done by disruption of the cells under liquid nitrogen using mortar and pistil and then th...
ORGANISM(S): Saccharomyces cerevisiae 
2008-12-11 | E-GEOD-12369 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of S. pombe meu5delta and wild type cells over-expressing the transcription factor mei4 to determine mRNA half-lives
The Meu5 RNA-binding protein stabilises its targets during meiosis. We used an in vivo labelling system (Cleary et al. 2005 Nat Biotechnol. 23, 232-7) to estimate the decay rates of meu5delta and wild type cells over-expressing the transcription factor Mei4.
ORGANISM(S): Schizosaccharomyces pombe 
2011-01-01 | E-TABM-776 | biostudies-arrayexpress
TranscriptomicsEffects of upf1delta on RNA stability
The Upf1 protein is a major factor in nonsense-mediated decay. We used an in vivo labelling system (Cleary et al. 2005 Nat Biotechnol. 23, 232-7) to estimate the decay rates of upf1delta and wild type cells over-expressing the transcription factor Mei4.
ORGANISM(S): Schizosaccharomyces pombe 
2013-06-03 | E-MTAB-1324 | biostudies-arrayexpress
UnknownOver-the-counter cough and cold medicines: reported poisonings of children before and after the 2012 and 2020 labelling changes in Australia.
Not available
S-EPMC10953310 | biostudies-literature
TranscriptomicsTesticular germ cell tumour cells release microRNA containing extracellular vesicles that induce phenotypic and genotypic changes in cells of the tumour microenvironment
Malignant germ-cell-tumours (GCTs) are characterised by microRNA (miRNA/miR-) dysregulation, with universal over-expression of miR-371~373 and miR-302/367 clusters regardless of patient age, tumour site, or subtype (seminoma/yolk-sac-tumour/embryonal carcinoma). These miRNAs are released into the bl...
ORGANISM(S): Homo sapiens 
2023-09-30 | E-MTAB-13322 | biostudies-arrayexpress
ProteomicsQuantitative comparison of E.huxleyi proteome in C-cell, N-cell, and over the diel cycle
Quantitatve, isobaric labelling based, proteomics was used to study changes in the whole cell E. huxleyi proteome over a 24 hour cycle, and comparing C-cells with N-cells.
ORGANISM(S): Emiliania huxleyi 
2023-04-25 | PXD027567 | Pride
Biomineralization Emiliania huxleyi Coccolithophore Haptophyte Coccolith Diel 
ProteomicsDeuterium oxide labeling methods for bulk and single muscle protein synthesis measures, and measures of integrated muscle protein synthesis and breakdown rates
This methodological pilot study explored the feasibility of an integrated tracer approach for simultaneous measurement of human skeletal muscle protein synthesis and breakdown during prolonged deuterium oxide (²H₂O) labelling. Four healthy men completed a five week protocol combining daily oral ²H₂O...
ORGANISM(S): Homo sapiens (Human) 
2026-09-07 | PXD078552 | Pride
Human Skeletal muscle Protein trunover 
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