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ProteomicsNHS-Ester Tandem Labeling in One Pot for 48-Plex Proteomics
Stable-isotope labeling strategies are extensively used for multiplex quantitative proteomics. Hybrid isotope labeling strate-gies that combine the use of isotopic mass difference labeling and isobaric tags can greatly increase sample multiplexity. In this work, we present a novel hybrid isotope lab...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD024780 | Pride
48-plex proteomics Tmtpro Mtraq 
UnknownSolution versus gas-phase modification of peptide cations with NHS-ester reagents.
Not available
S-EPMC3265610 | biostudies-literature
UnknownGas-phase intramolecular protein crosslinking via ion/ion reactions: ubiquitin and a homobifunctional sulfo-NHS ester.
Not available
S-EPMC3644013 | biostudies-literature
ProteomicsNHS esters are non-innocent protein acylating reagents
N-Hydroxysuccinimide (NHS)-ester derivatives represent one of the most widely used reagents in biological chemistry and chemical biology. Their perceived utility as acylating agents –intermediates that are sufficiently stable to be isolated (even in commercial form) yet active enough to allow direct...
ORGANISM(S): Escherichia coli Bacteria 
2025-04-27 | PXD056949 | Pride
Ring-opening reaction N-hydroxysuccinimide (nhs)-ester derivatives N-succinamide derivatives 
ProteomicsProtein Structure Determination in Biological Matrices by Mass Spectrometry and Computational Biology
We have implemented the use of a heterobifunctional, UV photoactivatable cross-linker, which greatly increases the number of identified cross-links compared with homobifunctional, NHS-ester based cross-linkers. We have cross-linked human serum albumin in the context of human blood serum. We present ...
ORGANISM(S): Homo sapiens (Human) 
2015-09-22 | PXD001692 | Pride
Cross-linking and mass spectrometry Human serum albumin 
TranscriptomicsPACAP dependency of genes regulated in adrenal gland
We investigated the effects of PACAP knock out on the transcriptosome profile of adrenal gland. A 35,546-clone mouse cDNA microarray was used to investigate transcriptional changes Keywords: transcript identification design In the hybridization method employed, equal amounts (2-5 ug/15.5 ul) of tota...
ORGANISM(S): Mus musculus 
2010-05-26 | E-GEOD-7405 | biostudies-arrayexpress
ProteomicsNHS Carbamate Cross-Linkers Provide Complementary XL-MS Restraints via Preferential N-Terminal Targeting
Cross-linking mass spectrometry (XL-MS) has become a valuable tool for investigating the structural morphology and plasticity of proteins. Traditional cross-linkers contain two N-hydroxy succinimide (NHS) esters that mainly react with lysine residues. In this work, we optimized the in-solution react...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2026-03-30 | PXD069252 | Pride
Crosslinking ms Cross-linking ms Dsso carbamate Nhs carbamate cross-linkers N-terminal reactivity Xl-ms Cross-linking mass spectrometry Ms-cleavable cross-linkers 
ProteomicsPTMC_A pyridinium-based strategy for bioorthogonal conjugation-assisted purification method for profiling cell surface proteome
Cell surface proteins (CSPs) are valuable targets for therapeutic agents, but achieving highly selective CSP enrichment in cellular physiology remains a technical challenge. To address this challenge, we propose a newly developed sulfo-pyridinium-ester (SPE) cross-linking probe, followed by two-step...
ORGANISM(S): Homo Sapiens 
PXD044541 |
TranscriptomicsRat Brain-Heart-Liver Control Mix Self-Self Hybridization
Eight “self-self” hybridizations were prepared as follows. Total RNA was isolated from each of three rat livers and brains, and equal mass amounts of each of these six samples were combined to create a mixed RNA population. Cy3- and Cy5-labeled cDNA was generated from this RNA mixture using an amino...
ORGANISM(S): Rattus norvegicus 
2007-09-11 | GSE8950 | GEO
TranscriptomicsReciprocal interaction of Wnt and RXR-α pathways in hepatocyte development and hepatocellular carcinoma
Genomic analysis of human hepatocellular carcinoma (HCC) is potentially confounded by the differentiation state of the hepatic cell-of-origin. Here we integrated genomic analysis of mouse HCC (with defined cell-of-origin) along with normal liver development. We found a major shift in expression o...
ORGANISM(S): Mus musculus 
2015-01-17 | E-GEOD-65063 | biostudies-arrayexpress
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