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Activation of oncogenes in healthy cells leads to a permanent cell cycle arrest termed oncogene-induced senescence (OIS) characterized by increased DNA damage and deep epigenetic resetting. Bypassing this premalignant state promotes tumorigenesis. Here, we show that cells entering OIS accumulate hig...
ORGANISM(S): Homo sapiens (Human) 
2026-04-27 | PXD060652 | Pride
Heterologous E. coli expression of AtABC1K6 tagged with maltose binding protein (MBP) and 6xHis tags revealed a spontaneous cleavage event of the recombinant protein during purification. Resulting bands were resolved on SDS-PAGE and excised for MS/MS identification. Mass spec-based identification of...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-03-02 | PXD030384 | Pride
In this study, we analyzed the impact of anoxia and re-aeration on tolerant rice at a proteomic level using two-dimensional gel electrophoresis followed by mass spectrometry. Mass spectrometry revealed 82 spots corresponding to 13 and 8 unique proteins in shoots and roots, respectively. Spot-wise cl...
ORGANISM(S): Oryza sativa (Rice) 
2025-09-29 | PXD067255 | Pride
Proteome comparison of isolated plastoglobules (PGs) from Arabidopsis thaliana (Col-0 ecotype) under unstress (UNS) and high light (HL) stress conditions. For both conditions, PGs were isolated from three independent set of plants each (i.e. three for UNS and three for HL). PGs were isolated from th...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-04-13 | PXD019735 | Pride
The completion of the Plasmodium falciparum clone 3D7 genome provides a basis on which to conduct comparative proteomics studies of this human pathogen. Here, we applied a high-throughput proteomics approach to identify new potential drug and vaccine targets and to better understand the biology of t...
ORGANISM(S): Plasmodium falciparum 3D7 
Gastric cancer (GC) is the third leading cause of cancer-related death worldwide, continuously requiring innovative target therapies. The sialyl-lewis A (SLeA) antigen offers tremendous potential for precise cancer targeting. This glycan is rarely expressed by healthy tissues and blood cells but is ...
ORGANISM(S): Homo sapiens (Human) 
2020-05-11 | PXD016968 | Pride
In chicken DT40 cells, there are six linker histone H1 variants and 12 of coding genes. We have previously reported of 11 out of 12 H1 knock out DT40 cells (Takami et al., Genes to Cell 1997 [PMID:9491804]) but complete H1 null DT40 cells could not established, so far. We identified one of the H1 va...
ORGANISM(S): Gallus gallus 
The in vivo labeling technique Split-Biotin identification (Split-BioID, De Munter et al., 2017, Schopp et al., 2017, Cho et al., 2020) was used to capture the common microenvironment of Bre5 and the ribosomal protein Rps2 (uS5). Biotinylated proteins were enriched from cell lysates using affinity p...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-11-10 | PXD028879 | Pride
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