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ProteomicsSUMOylation regulates chromatin protein network and accessibility at glucocorticoid receptor-binding sites
Glucocorticoid receptor (GR) is an essential transcription factor (TF), controlling metabolism, development and immune responses. SUMOylation regulates chromatin occupancy and target gene expression of GR in a locus-selective manner, but the mechanism of regulation has remained elusive. Here, we sho...
ORGANISM(S): Homo sapiens (Human) 
2021-09-08 | PXD015183 | Pride
Glucocorticoid receptor Proteomics Chromatin regulation Sumoylation Transcription factors 
ProteomicsSingle-minded homolog 2 resides in the chromatin protein network of androgen receptor and modulates gene expression in prostate cancer cells Single-minded homolog 2 resides in the chromatin protein network of androgen receptor and modulates gene expression in prostate cancer cells
We investigated the composition of chromatin protein network around endogenous androgen receptor (AR) in VCaP castration resistant prostate cancer cells using recently developed chromatin-directed proteomic approach called ChIP-SICAP . The androgen-induced AR chromatin protein network contained exp...
ORGANISM(S): Homo sapiens (Human) 
2021-06-17 | PXD025193 | Pride
Smarca4 Sim2 Castration-resistant prostate cancer cell Chip-sicap Chromatin accessibility Gene expression Protein-protein interactions Chromatin Androgen signaling Chromatin-mediated interaction 
ProteomicsIP of endogenous SUMO2/3 in HEK293 cells
Aim of the experiments was to identify endogenously SUMO2/3 modified proteins in HEK293 cells. Cells were treated with dexamethasone (dex) or vehicle (etoh) and with heatshock (+43C, HS) or normal growth temperature (+37C, non-HS). Cells were induced with tetracycline to express BirA*-GR (glucocorti...
ORGANISM(S): Homo sapiens (Human) 
2021-01-11 | PXD017714 | Pride
Sumo2/3 Heatshock Immunoprecipitation Hek293 
ProteomicsBioID of SUMOylation-deficient glucocorticoid receptor GR3KR
Purpose of these experiments was to assess the effect of glucocorticoid receptor SUMOylation on its protein-protein interactions using BioID. Experiments were performed with SUMOylation deficient GR3KR (K277,293,703R) fused to the BirA* biotin ligase tag.
ORGANISM(S): Homo sapiens (Human) 
2021-01-11 | PXD017713 | Pride
Human Glucocorticoid receptor Bira Gr Bioid Hek293 Sumo 
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