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ProteomicsPAM complex sequesters from TIM translocon and becomes part of an insoluble protein fraction under protein folding stress
Upon mitochondrial dysfunction, mitophagy has been described to be activated by a breakdown of membrane potential leading to PINK1 accumulation on the outer membrane and engulfment of mitochondria for degradation. However, recent findings have indicated that mitophagy may also be triggered in the ab...
ORGANISM(S): Homo sapiens (Human) 
2022-09-03 | PXD022524 | Pride
Human Mitophagy Hela cell line Pam complex Lc-ms/ms 
ProteomicsPAM complex sequesters from TIM translocon and becomes part of an insoluble protein fraction under protein folding stress
Upon mitochondrial dysfunction, mitophagy has been described to be activated by a breakdown of membrane potential leading to PINK1 accumulation on the outer membrane and engulfment of mitochondria for degradation. However, recent findings have indicated that mitophagy may also be triggered in the ab...
ORGANISM(S): Homo sapiens (Human) 
2022-09-03 | PXD022530 | Pride
Pam interactors Biotinylation-streptavidin-pull down Protein misfolding Turboid 
GenomicsRNA-guided Location and Function of the Cas9 CRISPR Endonuclease in Mammalian Cells
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated enzyme Cas9 is an RNA-guided nuclease that has been widely adapted for genome editing in eukaryotic cells. However, the in vivo target specificity of Cas9 is poorly understood and most studies rely on in silico predicti...
ORGANISM(S): Mus musculus 
2014-08-29 | E-GEOD-57650 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of porcine alveolar macrophages (PAM) after antibody-mediated crosslinking of sialoadhesin (Sn, Siglec-1)
In the present study, Sn expressed on primary pig macrophages was crosslinked with the anti-Sn monoclonal antibody 41D3 and the mRNA expression profiles were compared, using the Affymetrix microarray technology, to macrophages incubated with the irrelevant, isotype matched, monoclonal antibody 13D12
ORGANISM(S): Sus scrofa 
2007-03-09 | E-MEXP-991 | biostudies-arrayexpress
TranscriptomicsDeep sequencing data for PAM-SCANR tested CRISPR-Cas systems
In this work, we analyzed recognized PAM sequences from four CRISPR-Cas systems: E. coli I-E, B. halodurans I-C, S. thermophilus CR1 II-A, and F. novicida V. Cells containing functional PAMs were sorted using FACS and subsequently sequenced. Ranking of recognized PAMs from a positive screen
ORGANISM(S): Francisella tularensis subsp. novicida 
2016-02-17 | E-GEOD-75718 | biostudies-arrayexpress
UnknownPAM forms an atypical SCF ubiquitin ligase complex that ubiquitinates and degrades NMNAT2.
Not available
S-EPMC6130950 | biostudies-literature
OtherExpanding the Scope of Bacterial CRISPR Activation with PAM-flexible dCas9 Variants
CRISPR-Cas transcriptional tools have been widely applied for programmable regulation of complex biological networks. In comparison to eukaryotic systems, bacterial CRISPR activation (CRISPRa) has stringent target site requirements for effective gene activation. While genes may not always have an NG...
ORGANISM(S): Homo sapiens 
2022-11-15 | GSE217559 | GEO
UnknownPAM-readID is a rapid, simple, and accurate PAM determination method for CRISPR-Cas enzymes in mammalian cells.
Not available
S-EPMC12627570 | biostudies-literature
UnknownThe PAM domain, a multi-protein complex-associated module with an all-alpha-helix fold.
Not available
S-EPMC319699 | biostudies-literature
GenomicsExpanding the Scope of Bacterial CRISPR Activation with PAM-flexible dCas9 Variants
Expanding the Scope of Bacterial CRISPR Activation with PAM-flexible dCas9 Variants
PRJNA899546 | ENA
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