Uncovering dark mass in population proteomics: Pan-analysis of Single Amino-acid Polymorphism (Pan-SAP) revealed quantitative relevance with cognition and aging
Excessive fat deposition can trigger metabolic diseases, and it is crucial to identify factors that can break the link between fat deposition and metabolic diseases. Healthy obese Laiwu pigs (LW) are high in fat content but resistant to metabolic diseases. In this study, we compared the fecal microb...
Numerous RNA-binding proteins are arginine methylated and inhibition of arginine methylation triggers changes in alternative polyadenylation in cancer cells. Therefore we aimed to investigate whether this phenotype was also triggered in an untransformed cell line - RPE-1. RPE-1 cells were treated wi...
We report the identification of 67 previously undescribed histone modifications, increasing the current number of known histone marks by about 70%. We further investigated one of the marks, lysine crotonylation (Kcr), confirming that it represents an evolutionarily-conserved histone posttranslationa...
Cleavage and polyadenylation factors PCF11 and CSTF2 are arginine methylation substrates. Therefore we aimed to study how the alternative polyadenylation changes caused by arginine methylation inhibition are affected by overexpression of either PCF11 or CSTF2. We generated LU-99 cell lines expressin...
Despite advances in the development of direct KRAS inhibitors, KRAS-mutant cancers continue to exhibit resistance to the currently available therapies. Here, we identified REGγ as a mutant KRAS-associated factor that enhances REGγ transcription through the KRAS intermediate NRF2, suggesting that the...
Numerous RNA-binding proteins involved in alternative polyadenylation are arginine methylation substrates. Therefore we aimed to study how alternative polyadenylation is impacted by preventing arginine methylation, and how this phenotype develops at various time points. We treated LU-99 cells with 1...
