Sort   by:  
 Page size 
ProteomicsDissecting the secretory pathway with electrophoresis reveals intra-Golgi trafficking pathways and enables compilation of sub-Golgi and sub-ER proteomes.
The Golgi is the hub of the eukaryotic secretory pathway, trafficking proteins and lipids, as well as synthesizing complex sugars. Different biosynthetic reactions are associated with different compartments of its complex architecture. Although pre- and post-Golgi trafficking has been much studied...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-09-11 | PXD004596 | Pride
Arabidopsis Free flow electrophoresis Proteomics Trans-membrane span Secretory pathway Polysaccharide Endoplasmic reticulum Trafficking Golgi Cisternae Immunogold 
ProteomicsA novel tool, Multiple Marker Abundance Profiling, uses Selected Reaction Monitoring and data-dependent acquisition for rapid estimation of organelle abundance in subcellular samples.
Methods were investigated that would deliver rapid and semi-quantitative analysis of the subcellular composition of Arabidopsis samples, as the standard technique for this i.e. immunoblotting is difficult to quantify and relies on a very few protein targets, whose responses to developmental or envir...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-26 | PXD005408 | Pride
Srm Subcellular proteomics Organelle Arabidopsis Quantitation Shotgun 
ProteomicsProteomic analysis of separated Golgi cisternae gives insights into secretory pathway organization Proteomic analysis of separated Golgi cisternae gives insights into secretory pathway organization
The Golgi apparatus is the hub of the secretory pathway, trafficking proteins and lipids and synthesizing complex glycans. The spatial distribution of resident proteins across individual Golgi cisternae is functionally essential for complex molecule biosynthesis. However, the inability to-date to se...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-09-11 | PXD009978 | Pride
Proteomic analysis of separated golgi cisternae gives insights into secretory pathway organization 
TranscriptomicsTranscription profiling of monocytes derived from tolerant (Sahiwals, Bos indicus) and susceptible (Holstein-Friesians, Bos taurus) cattle to in vitro infection with Theileria annulata
Tropical theileriosis in a cattle disease of global economic importance, caused by the tick-borne protozoan parasite Theileria annulata. Conventional control strategies are failing to contain the disease and an attractive alternative is the use of pre-existing genetic resistance or tolerance. Howe...
ORGANISM(S): Bos taurus 
2007-04-26 | E-MAXD-25 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of bovine monocytes stimulated with with interferon-g and lipopolysaccharide for 2 or 16 hours to validate a bovine macrophage specific cDNA microarray
Background The response of macrophages to danger signals is an important early stage in the immune response. Our understanding of this complex event has been furthered by microarray analysis, which allows the simultaneous investigation of the expression of large numbers of genes. However, the mic...
ORGANISM(S): Bos indicus 
2006-09-20 | E-MAXD-16 | biostudies-arrayexpress
UnknownIntracellular processing of immunostimulatory CpG-siRNA: Toll-like receptor 9 facilitates siRNA dicing and endosomal escape.
Not available
S-EPMC3742675 | biostudies-literature
ProteomicsA novel tool, Multiple Marker Abundance Profiling, uses Selected Reaction Monitoring and data-dependent acquisition for rapid estimation of organelle abundance in subcellular samples.
Methods were investigated that would deliver rapid and semi-quantitative analysis of the subcellular composition of Arabidopsis samples, as the standard technique for this i.e. immunoblotting is difficult to quantify and relies on a very few protein targets, whose responses to developmental or envir...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-03-02 | PXD004576 | Pride
Srm Subcellular proteomics Organelle Arabidopsis Quantitation Shotgun 
MetabolomicsRA-MAP: mapping the molecular immunological landscape in early rheumatoid arthritis with vaccine controls

Rheumatoid arthritis (RA) is a chronic inflammatory disorder with poorly defined aetiology characterised by synovial inflammation with variable disease severity and drug responsiveness. To investigate the peripheral blood immune cell landscape of RA, we performed comprehensive clinical and molecu...

2021-06-16 | MTBLS1497 | MetaboLights
TranscriptomicsHuman lung adenocarcinoma DNA copy number profiling
Lung adenocarcinoma’s genomic alterations exhibit vast variation. Lung adenocarcinoma also has previously described intrinsic gene expression subtypes (Hayes et al. 2006, PMID: 17075127). This study’s aim was to detect and validate subtype-specific alterations. A portion of these specimens were a...
ORGANISM(S): Homo sapiens 
2012-05-16 | E-GEOD-36363 | biostudies-arrayexpress
UnknownMotor patterns of the small intestine explained by phase-amplitude coupling of two pacemaker activities: the critical importance of propagation velocity.
Not available
S-EPMC4572367 | biostudies-literature
Sort   by:  
 Page size