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ProteomicsCharacterizing the dynamic oligomerization of Hsp90β using photo-cross-linking
Cross-linking mass spectrometry (XL-MS) is a powerful tool for probing protein structures. While conventional chemical cross-linkers react with specific residues with defined chemistry, photo-cross-linkers, despite their superior reactivity, have been hindered by incomplete mechanistic understanding...
ORGANISM(S): Homo sapiens (Human) 
2026-04-09 | PXD072009 | Pride
Hsp90 Cleavable cross-linkers Diazirine Photo-cross-linking 
ProteomicspLink Characterizing the dynamic oligomerization of HSP90β using photo-cross-linking
Cross-linking mass spectrometry (XL-MS) is a transformative tool for probing protein structures. While conventional chemical crosslinkers exhibit well-defined chemistry for specific residues, photo-crosslinkers, despite their superior reactivity, have been hindered by incomplete mechanistic understa...
ORGANISM(S): Homo sapiens (Human) 
2026-04-09 | PXD066889 | Pride
Cleavable cross-linkers Diazirine Photo-cross-linking Machine learning (decision tree) Hsp90β 
ProteomicsCross-linking mass spectrometry for protein structure analysis with homo-bifunctional cross-linkers
Cross-linking mass spectrometry for protein structure analysis with homo-bi-functional photo-activatable cross-linkers. Cross-linkers were applied to BSA, importin-alpha/beta, and dengue NS2b-NS3 protease protein complexes.
ORGANISM(S): Homo Sapiens (human) Cellular Organisms 
2026-01-05 | PXD067180 | JPOST Repository
ProteomicsIdentification of HJURP binding determinants on the Mis18 complex using photo-cross-linking and mass spectrometry
In the early G1 phase of the cell cycle, human HJURP is recruited to centromeres by the Mis18 complex, which consists of Mis18α, Mis18β and M18BP1. This interaction of HJURP with the Mis18 complex is essential for CENP-A deposition and the preservation of centromere identity. To identify the binding...
ORGANISM(S): Homo sapiens (Human) 
2019-08-01 | PXD013339 | Pride
Photo-cross-linking Q-exactive Abk Human Kinetochore Centromere Protein complex Bpa Stavrox 
ProteomicsCombining Affinity Enrichment, Cross-Linking with Photo-Amino Acids, and Mass Spectrometry for Probing Protein Kinase D2 Interactions
We present an approach that relies on the affinity enrichment of a target protein from a complex mixture, followed by a UV-induced activation of incorporated diazirine photo-reactive amino acids (photo-methionine and photo-leucine) and a covalent fixation of interaction partners. The captured protei...
ORGANISM(S): Homo sapiens (Human) 
2017-04-10 | PXD005349 | Pride
Pkd2 Affinity enrichment Protein interaction Photo-cross-linking Photo-methionine Photo-leucine Orbitrap fusion 
ProteomicsDeciphering Diazirine Reaction Mechanism: through Irradiation Modulation for Residue-Specific Distance Restraints in Protein Photo-Crosslinking
The diazirine photoreactive group has been widely used for photo-labeling and photo-cross-linking (PXL) of proteins. Yet, due to the mechanistic complexity of diazirine photo-reaction, site-specific and quantitative analysis of protein PXL data remains challenging. Herein, we have built an in-line p...
ORGANISM(S): Bos taurus (Bovine) 
2024-06-22 | PXD048452 | Pride
Photo cross-linking Multiple reaction monitoring Optical power density Distance restraints Alkyl diazirine 
UnknownAutomatic Photo-Cross-Linking System for Robotic-Based In Situ Bioprinting.
Not available
S-EPMC10716819 | biostudies-literature
UnknownIncreasing sensitivity of antibody-antigen interactions using photo-cross-linking.
Not available
S-EPMC10326447 | biostudies-literature
UnknownGenetically Encoded 2-Aryl-5-carboxytetrazoles for Site-Selective Protein Photo-Cross-Linking.
Not available
S-EPMC5423124 | biostudies-literature
UnknownMetabolism of diazirine-modified N-acetylmannosamine analogues to photo-cross-linking sialosides.
Not available
S-EPMC3178686 | biostudies-literature
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