Photoaffinity label

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1Department of Chemistry, University of Konstanz, Konstanz, Germany 2Konstanz Resea1Department of Chemistry, University of Konstanz, Konstanz, Germany 2Konstanz Research School-Chemical Biology, University of Konstanz, Konstanz, Germany (1)
Analytics Core Facility Essen (ACE), Chemische Biologie, Universität Duisburg-Essen, ZMB, Germany (1)
Basic Immune System of Plants / Protein Mass Spectrometry Max Planck Institute for Plant Breeding Research Carl-von-Linne-Weg 10, 50829 Cologne, Germany (1)
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Department of Pharmaceutical Chemistry and BioanalyticsInstitute of Pharmacy Martin Luther University Halle-Wittenberg Wolfgang-Langenbeck-St. 4 D-06120 Halle (Saale), Germany (1)
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Proteome Dynamics Lab, Max Delbrueck Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany (1)
Schuler Assistant Professor In Translational Organic Chemistry, School of Chemistry, Trinity College Dublin, Republic of Ireland (1)
State Key Laboratory of Microbial Metabolism, Shanghai-Islamabad-Belgrade Joint Innovation Center on Antibacterial Resistances, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. (1)
Technical University of Munich, Department of Chemistry, Chair of Organic Chemistry 2, Germany (1)
Technische Universität München Chair of Organic Chemistry II Center for Integrated Protein Science (CIPSM) Lichtenbergstrasse 4 85748 Garching, Germany (1)
The Plant Chemetics Laboratory, Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, United Kingdom (1)
University of Constance, Department of Chemistry (1)
dept of Bioorganic synthesis, Leiden Institute for Chemistry, Leiden University, The Netherlands (1)

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Design and evaluation of a cyclobutane diazirine alkyne tag for photoaffinity labeling in cells

Alkyl diazirines are frequently used in photoaffinity labeling to trap and identify small molecule–protein interactions. How-ever, the alkyl diazirine can preferentially label acidic amino acids and acidic protein surfaces in a pH-dependent manner, presumably via a long-lived alkyl diazo intermediat...

ORGANISM(S): Homo sapiens (Human)

2024-01-04 | PXD035954 | Pride