Photoaffinity-labeling

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Design and evaluation of a cyclobutane diazirine alkyne tag for photoaffinity labeling in cells

Alkyl diazirines are frequently used in photoaffinity labeling to trap and identify small molecule–protein interactions. How-ever, the alkyl diazirine can preferentially label acidic amino acids and acidic protein surfaces in a pH-dependent manner, presumably via a long-lived alkyl diazo intermediat...

ORGANISM(S): Homo sapiens (Human)

2024-01-04 | PXD035954 | Pride