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ProteomicsHAPI31-IP-MS, containing PI31 truncations and Parkinson's associated variants, with or without FBXO7
Triplicate HA-Immunoprecipitations of HA-tagged PI31 variants including full length (1-271), FP domain (1-151) and Parkinson's associated sequence variants, L53M, L228V, R231X(1-230), R242G, R242C, overexpressed in FBXO7-/- PSMF1-/- HEK293T cells co-overexpressed with full length FBXO7(1-522), or wi...
ORGANISM(S): Homo sapiens (Human) 
2026-06-22 | PXD077016 | Pride
Fbxo7 Parkinson's disease Human Keywords: pi31 Ip-ms 
ProteomicsSEC16A forms a complex with PI31 and TBK1
To identify interaction partners of PI31, we immunoprecipitated PI31 from NOD2 and TLR2 stimulated DCs, followed by performing tryptic digestion and mass spectrometry analysis.
ORGANISM(S): Homo sapiens (Human) 
2026-05-28 | PXD013249 | Pride
Pi31  sec16a  tbk1 
ProteomicsTBK1 mediates NOD2 and TLR2 mediated PI31 phosphorylation
We sought to identify the residue on which PI31 is phosphorylated following NOD2 and TLR2 activation. We immunoprecipitated endogenous PI31 from cellular extracts of THP1 cells stimulated with MDP and PAM3CSK4, and then conducted LC-MS/MS analysis on the immunoprecipitated material.
ORGANISM(S): Homo sapiens (Human) 
2026-05-28 | PXD013248 | Pride
Pi31 Tbk1 Phosphorylation 
ProteomicsPI31 modulates the quantity of MHC class I loaded peptides in DCs
We used siRNAs to knockdown PI31 in primary DCs, followed by capturing HLA class I complexes using W6/32-conjugated immunoresin. Acid treatment abolished noncovalent interactions among the complex components. Using reverse-phase HPLC, we separated eluted peptides from the α-chain and β2-microglobuli...
ORGANISM(S): Homo sapiens (Human) 
2026-05-28 | PXD013247 | Pride
Pi31 Proteasome Antigen presentation Mhc class i 
UnknownPI31 is a positive regulator of 20S immunoproteasome assembly.
Not available
S-EPMC12148024 | biostudies-literature
UnknownPI31 is a positive regulator of 20S immunoproteasome assembly.
Not available
S-EPMC11761684 | biostudies-literature
UnknownPI31 expression is neuroprotective in a mouse model of early-onset parkinsonism.
Not available
S-EPMC12478140 | biostudies-literature
ProteomicsFLAG-IP, FLAG-FBXO7 samples with either full-length or C-terminally truncated FBXO7 expressed with or without PI31 present
Triplicate FLAG-Immunoprecipitations of FLAG-tagged FBXO7 overexpressed in FBXO7-/- PSMF1-/- HEK293T cells co-overexpressed with full length PI31(1-271), PI31∆PRR (1-151), or without PI31 (empty vector). wild type HEK293T containing FLAG-tagged eGFP samples represent a negative control IP Identities...
ORGANISM(S): Homo sapiens (Human) 
2026-06-22 | PXD077448 | Pride
Parkinson's disease Human Ip-ms Fbxo7 Pi31 
UnknownPI31 Is an Adaptor Protein for Proteasome Transport in Axons and Required for Synaptic Development.
Not available
S-EPMC6702053 | biostudies-literature
UnknownΗigh-resolution structure of mammalian PI31-20S proteasome complex reveals mechanism of proteasome inhibition.
Not available
S-EPMC10319324 | biostudies-literature
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