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ProteomicsProtein Analysis of the Tomato Male Gametophyte Following Heat-stress Exposure and Pre-treatment with an Ethylene-releaser Conferring Pollen Thermotolerance
Heat stress is a major cause for yield loss in many crops, including vegetable crops. Even short waves of high temperature, becoming more frequent during recent years, can be detrimental. Pollen development is most heat-sensitive, being the main cause for reduced productivity under heat-stress acros...
ORGANISM(S): Solanum lycopersicum 
2019-11-12 | PXD008283 | Pride
Tomato Pollen Ethylene Heat stress Shotgun proteomics 
ProteomicsHeat stress induced proteomes in tomato pollen mother cells and microspores
During the reproductive stage, tomatoes require nighttime temperatures below 21°C and daytime temperatures below 32°C for healthy flower development and fruit set. Prolonged exposure to temperatures above the optimal range can cause abnormalities in pollen development, leading to reduced fertility o...
ORGANISM(S): Solanum lycopersicum 
2025-05-07 | PXD060801 | Pride
Laser capture microdissection Pollen heat stress Tmt proteomics Tomato free microspores 
ProteomicsThe coupling of transcriptome and proteome adaptation during development and heat stress response of tomato pollen
Pollen development is central for plant reproduction and is assisted by changes of the transcriptome and the proteome. At the same time, pollen is largely heat sensitive, particularly at early developmental stages. To define the changes during development and the response of different stages to elev...
ORGANISM(S): Solanum lycopersicum 
2015-05-15 | PXD008762 | Pride
Pollen development Proteomics Heat stress Transcriptomics 
TranscriptomicsSmall noncoding RNAs in tomato pollen
We used tomato pollen in order to identify pollen stage-specific small non-coding RNAs (sncRNAs) and their target mRNAs. We further deployed elevated temperatures to discern stress responsive sncRNAs. For this purpose high throughput sncRNA-sequencing was performed for three-replicated sncRNAs libra...
ORGANISM(S): Solanum lycopersicum 
2015-08-20 | E-MTAB-3830 | biostudies-arrayexpress
ProteomicsGenome survey sequencing of Gossypium robinsonii to investigate the molecular responses of wild cotton pollen to extreme heat
Wild species are valuable resources for developing resilient crops to environmental stresses. We used Gossypium robinsonii, Australian wild cotton, to investigate the molecular signatures contributing to the tolerance of this plant to harsh environments. Three stages of pollen development, including...
ORGANISM(S): Gossypium robinsonii 
2022-02-10 | PXD027097 | Pride
Genome sequencing Wild cotton Swath-ms Pollen Heat stress 
ProteomicsBrief exposure to heat differentially affects development of male gametophytes in cotton (Gossypium hirsutum)
The development of viable gametes is especially susceptible to heat in all higher plants, including cotton (Gossypium hirsutum cv. Sicot 71), resulting in substantial reduction in lint quantity and quality at temperatures above 32 °C. Male reproductive cells are especially vulnerable to heat. This s...
ORGANISM(S): Gossypium hirsutum 
2020-10-19 | PXD015721 | Pride
Lc-msms Pollen development Heat stress Cotton 
TranscriptomicsElucidation of stress memory inheritance through epigenome alterations in Brassica rapa plants [RNA-seq]
In the current study we have profiled changes in the transcriptome of somatic and reproductive tissues of B.rapa plants following heat shock stress exposure. For this we used Illumina GAIIx platform. We have demonstrated that the highest tissue-specific alterations in the transcriptome profile are d...
ORGANISM(S): Brassica rapa 
2014-12-04 | E-MTAB-4131 | biostudies-arrayexpress
ProteomicsMolecular analyses of cotton (Gossypium hirsutum) identifies differential sensitivity to heat during stages of pollen development
Heatwaves resulting from global warming are a leading threat to plants because they specifically impair reproductive (gametophytic) development. We investigated pollen development using proteomics after exposure to heat (38/28 °C; day/night). Sampling enabled the identification of the impact of heat...
ORGANISM(S): Gossypium hirsutum 
2021-12-01 | PXD019873 | Pride
Tetrads Swath Uninucleate microspores Heat stress Anthesis Cotton Binucleate microspores 
TranscriptomicsHsfA2 controls the activity of developmentally and stress-regulated heat stress protection mechanisms in tomato male reproductive tissues
Male reproductive tissues are more sensitive to heat stress compared to vegetative tissues, however the basis of this phenomenon is poorly understood. Heat stress transcription factors (Hsfs) regulate the transcriptional changes required for protection and recovery from heat stress. HsfA2 has been c...
ORGANISM(S): Solanum lycopersicum 
2016-02-22 | E-GEOD-68500 | biostudies-arrayexpress
ProteomicsHeat-induced proteomes in tomato pollen mother cells
Tomato pollen production and viability is highly vulnerable to higher temperature. Hot summers with temperature reaching above 32°C can disrupt production of viable pollens and fruit set, resulting in yield loss. In recent years, temperature above 35-38oC has become a norm during mid-summer with po...
ORGANISM(S): Solanum lycopersicum 
2022-04-07 | PXD018915 | Pride
Tmt proteomics Pollen mother cells Laser capture microdissection Heat stress project description 
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