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TranscriptomicsTranscription profiling of Arabidopsis pollen and semi in vivo- and in vitro-grown pollen tubes.
Pollen tubes extend through pistil tissues and are guided to ovules where they release sperm for fertilization. Although pollen tubes can germinate and elongate in a synthetic medium, their trajectory is random and their growth rates are slower compared to growth in pistil tissues. Furthermore, inte...
ORGANISM(S): Arabidopsis thaliana 
2009-08-15 | E-GEOD-17343 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Arabidopsis germinating pollen
In sexual reproduction, a proper communication and cooperation between male and female organs and tissue are essential for male and female gametes to unite. In flowering plants, female sporophytic tissues and gametophyte direct a male pollen tube towards an egg apparatus, which consists of an egg ce...
ORGANISM(S): Arabidopsis thaliana 
2009-08-09 | E-GEOD-12316 | biostudies-arrayexpress
TranscriptomicsTranslatome of floral buds, in vivo pollinated pollen tubes, and in vitro cultured pollen grains/tubes from LAT52-HF-RPL18 Arabidopsis
Little is known of the transcriptome of in vivo-grown pollen tubes, due to the difficulty of collection of pollen tubes elongating within the maternal gynoecium.We obtained the mRNAs undergoing translation (the translatome) of in vivo-grown pollen tubes from self-pollinated gynoecia of Arabidopsis t...
ORGANISM(S): Arabidopsis thaliana 
2014-02-01 | E-GEOD-43299 | biostudies-arrayexpress
ProteomicsComprehensive cell-specific protein analysis in early and late pollen development from diploid microsporocytes to pollen tube growth
Pollen development in angiosperms is one of the most important processes controlling plant reproduction and thus productivity. At the same time, pollen development is highly sensitive to environmental fluctuations, including temperature, drought, and nutrition. Therefore, pollen biology is a major f...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2023-10-09 | PXD045970 | Pride
Microspores Pollen tubes Development Tobacco Pollen 
TranscriptomicsHigh-throughput sequencing of Arabidopsis thaliana pollen cDNA uncovers novel transcription and alternative splicing
Pollen grains of Arabidopsis thaliana contain two haploid sperm cells enclosed in a haploid vegetative cell. Upon germination, the vegetative cell extrudes a pollen tube that carries the sperm to an ovule for fertilization. Knowing the identity, relative abundance, and splicing patterns of pollen tr...
ORGANISM(S): Arabidopsis thaliana 
2013-05-25 | E-MTAB-4242 | biostudies-arrayexpress
ProteomicsNicotiana tabacum pollen tube lipid droplet proteome
The number of known proteins associated with plant lipid droplets (LDs) is small compared to other organelles. Many questions of LD biosynthesis and degradation remain open, also due to lack of candidate LD proteins whose characterization could help to elucidate their function in those processes. We...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2018-08-13 | PXD009184 | Pride
Tobacco Pollen tubes Lipid droplets 
TranscriptomicsTranscript Profiling of Maize Embryo Sac and Preliminary Identification of Genes Involved in Embryo Sac-Pollen Tube Interactions
In this study, we identified the genes specifically/preferentially expressed in maize embryo sac and genes differentially expressed before and after pollination in maize embryo sac via RNA-seq. In total, more than 30% of them are unknown functions and more than 80% of which are first reported in mai...
ORGANISM(S): Zea mays 
2015-04-25 | E-GEOD-57075 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Arabidopsis pollen germination and tube growth
Pollen germination, along with pollen tube growth, is an essential process for the reproduction of flowering plants. The germinating pollen with tip-growth characteristics provides an ideal model system for the study of cell growth and morphogenesis. As an essential step towards a detailed underst...
ORGANISM(S): Arabidopsis thaliana 
2008-10-25 | E-GEOD-6696 | biostudies-arrayexpress
TranscriptomicsRNA-seq of Solanum lycopersicum pollen tube at different germination temperatures. EVOREPRO dataset
Tomato pollen tube RNAseq data
ORGANISM(S): Solanum lycopersicum 
2021-04-14 | E-MTAB-9725 | biostudies-arrayexpress
ProteomicsProteomic analysis of secretions from pollen tubes and ovaries of Camellia oleifera cultured semi-in vivo
Self-inhibition of pollen tubes plays a key role in SI, but the underlying mechanism in Camellia oleifera is poorly understood. Collection of secreted proteins from Camellia oleifera pollen tubes and ovaries for high-throughput sequencing.
ORGANISM(S): Camellia oleifera 
2023-03-11 | PXD035406 | Pride
Proteome Camellia oleifera 
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