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ProteomicsSystematic investigation of protein phosphorylation in biomolecular condensates
We have adapted a recently developed quantitative proteomics based approach to measure protein solubility following a lysate centrifugation assay to determine the proportion of a protein in distinct subpopulations in a proteome-wide manner (Sridharan et al., 2019). Further, by combining the solubili...
ORGANISM(S): Homo sapiens (Human) 
2022-08-09 | PXD027769 | Pride
Protein solubility Phosphorylation Biomolecular condensate 
UnknownTl(2)Mo(9)Se(11).
Not available
S-EPMC3007497 | biostudies-literature
ProteomicsThermal proteome profiling of phosphorylated proteoforms
We combined thermal proteome profiling (TPP) with phosphopeptide enrichment to study the thermostability of phosphorylated proteoforms.
ORGANISM(S): Homo sapiens (Human) 
2021-01-04 | PXD015993 | Pride
Phosphopeptides Meltome Phosphoproteomics Thermal proteome profiling 
ProteomicsPhosphoProtein-Peptide Turnover Profiling, exploratory
We combine pulsed stable isotope labeling of amino acids (pSILAC) with enrichment of phosphorylated peptides in a method we term PhosphoProtein-Peptide Turnover Profiling (PhosphoPPToP). We apply the method to HeLa cells to find peptides whose clearance rates differ from the protein median. Using th...
ORGANISM(S): Homo sapiens (Human) 
2022-12-02 | PXD033254 | Pride
Post-translational modification Protein maturation Protein turnover Phosphorylation Mass spectrometry 
ProteomicsA genetic analysis reveals novel histone residues required for transcriptional reprogramming upon stress
Cells have the ability to sense, respond and adapt to environmental fluctuations. Stress causes a massive reorganization of the transcriptional program. Many examples of histone post-translational modifications (PTMs) have been associated with transcriptional activation or repression under steady-st...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-05-27 | PXD017134 | Pride
Phosphoproteomics Histone Ptms 
ProteomicsDeep quantitative glycoproteomics reveals gut microbiome induced remodeling of the brain glycoproteome
Protein glycosylation is a highly diverse post-translational modification, modulating key cellular processes such as cell signaling, adhesion and cell-cell interactions. Its deregulation has been associated with various pathologies, including cancer and neurological diseases. Methods capable of quan...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2025-02-07 | PXD052447 | Pride
Glycoproteomics Tmt Microbiome 
UnknownThe Chevrel phase HgMo(6)S(8).
Not available
S-EPMC2977540 | biostudies-literature
ModelsHammaren-Geissen2022_PPToP_Model12
The model encodes the general biological process of protein synthesis and post-translational modification (PTM, such as protein phosphorylation), viewed through the eyes of a specific, widely-used experimental method. Specifically, we model measurements of pulsed stable isotope labelling of amino ac...
2024-09-02 | BIOMD0000001078 | BioModels
ProteomicsA vascular phosphoproteome atlas unveils a functional Tie‑Wnt signalling axis in the liver
Single cell transcriptomic analyses (scRNAseq) of hepatocytes and liver endothelial cells (L-EC) have revolutionized the understanding of the spatial architecture of liver structure and function. The spatial alignment of L-EC and hepatocytes is pivotal for liver function in health and disease given ...
ORGANISM(S): Mus musculus (Mouse) 
2021-05-21 | PXD020805 | Pride
Wnt Vasculature Liver Phospho-proteome Endothelial Zonation Tie 
UnknownNudibranch predation boosts sponge silicon cycling.
Not available
S-EPMC9859830 | biostudies-literature
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