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ProteomicsEffect of Nop53 depletion on the compositional changes during pre-60S maturation
The eukaryotic ribosome biogenesis is a highly orchestrated multistep process that starts at the nucleolus with the transcription of pre-rRNAs 5S and 35S. The latter comprises the mature 18S, 5.8S and 25S rRNAs separated by internal transcribed spacers (ITS1 and ITS2) and externally flanked by the 5...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-09-09 | PXD023383 | Pride
Nop53 Eukaryotic ribosome biogenesis Pre-60s S. cerevisiae 
ProteomicsCross-Linking Mass Spectrometry (CXMS) of human pre-60S ribosomal particles
Cross-Linking Mass Spectrometry (CXMS) analysis of human pre-60S ribosomal particles.
ORGANISM(S): Homo Sapiens 
2020-05-07 | PXD019025 |
ProteomicsRbp95 binds 25S rRNA helix H95 and cooperates with the Npa1 complex during early pre-60S particle maturation
Eukaryotic ribosome synthesis involves more than 200 assembly factors, which promote ribosomal RNA (rRNA) processing, modification and folding, and assembly of ribosomal proteins. The formation and maturation of the earliest pre-60S particles requires structural remodeling by the Npa1 complex, but i...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-10-13 | PXD030106 | Pride
Ribosome assembly factor Yeast S. cerevisiae Tandem-affinity purification (tap) Npa1 Pre-60s particles Rbp95 
UnknownEmergence of the primordial pre-60S from the 90S pre-ribosome.
Not available
S-EPMC8994135 | biostudies-literature
ProteomicsDiverse roles of assembly factors revealed by structures of late nuclear pre-60S ribosomal particles
Ribosome biogenesis is a highly complex process in eukaryotes, involving temporally and spatially regulated ribosomal protein (r-protein) binding and rRNA remodeling events in the nucleolus, nucleoplasm and cytoplasm. Hundreds of assembly factors (AFs), organized into sequential functional groups, f...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-06-01 | PXD003736 | Pride
Assembly factors Cryo-em Pre-60s ribosome Cxms 
UnknownPuf6 primes 60S pre-ribosome nuclear export at low temperature.
Not available
S-EPMC8338941 | biostudies-literature
ProteomicsNuclear VCP-like 2 (NVL2)-associated complex
LC-MSMS analysis of NVL2-associated pre-60S complex. To obtain more insights into the molecular mechanism of this effects caused by the mutant NVL2 in pre-60S complexes,we searched for additional proteins that preferentially interact with the NVL2(E365Q/E682Q) in comparison with wild-type NVL2. Co-m...
ORGANISM(S): Homo Sapiens (human) 
2025-01-31 | PXD057741 | JPOST Repository
TranscriptomicsCircular non-coding RNA ANRIL modulates ribosomal RNA maturation and atherosclerosis in humans.
Circular RNAs (circRNAs) are broadly expressed in eukaryotic cells, but their role in human health and disease remains obscure. Here, we show that circular antisense non-coding RNA in the INK4 locus (circANRIL), which is transcribed at a locus of atherosclerotic cardiovascular disease on chromosome ...
ORGANISM(S): Homo sapiens 
2016-08-22 | E-GEOD-65392 | biostudies-arrayexpress
ProteomicsThe RNA helicase Dbp10 coordinates assembly factor association with PTC formation during ribosome biogenesis
During ribosome biogenesis a plethora of assembly factors and essential enzymes drive the unidirectional maturation of nascent pre-ribosomal subunits. The DEAD-box RNA helicase Dbp10 is suggested to restructure pre-ribosomal rRNA of the evolving peptidyl-transferase center (PTC) on nucleolar ribosom...
ORGANISM(S): Saccharomyces cerevisiae Saccharomyces cerevisiae (Baker's yeast) 
2024-05-22 | PXD046068 | Pride
Ribosome assembly. pre-60s Assembly factors S. cerevisiae 
ProteomicsStructure of the AAA-ATPase Drg1 during extraction of Rlp24 from pre-60S particles
The AAA-ATPase Drg1 is a key factor in eukaryotic ribosome biogenesis that initiates cytoplasmic maturation of the large ribosomal subunit. Drg1 releases the shuttling maturation factor Rlp24 from pre-60S particles shortly after nuclear export, a strict requirement for downstream maturation. The mol...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-10-14 | PXD032098 | Pride
Large ribosomal subunit Ribosome biogenesis Aaa-atpases Substrate recognition Substrate translocation mechanism Single particle cryo-em 
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