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TranscriptomicsBulk RNA Barcoding and sequencing (BRB-seq) protocol for RNA-seq of human pre-adipocytes and differentiated adipocytes
Here, we validate a novel protocol, Bulk RNA Barcoding and sequencing (BRB-seq), that combines the multiplexing-driven cost-effectiveness of a single-cell RNA-seq protocol with the efficiency of a bulk RNA-seq procedure. For this we use BRB-seq protocol on human pre-adipocytes and differentiated adi...
ORGANISM(S): Homo sapiens 
2018-02-06 | E-MTAB-6469 | biostudies-arrayexpress
TranscriptomicsSCRB-seq comparison with TruSeq on bulk RNA-seq human LCL (DMSO) and BAY treated LCL (BAY)
Here, we validate a novel protocol, Bulk RNA Barcoding and sequencing (BRB-seq), that combines the multiplexing-driven cost-effectiveness of a single-cell RNA-seq protocol with the efficiency of a bulk RNA-seq procedure. For this we first gauge the applicability of the SCRB-seq protocol on bulk DMSO...
ORGANISM(S): Homo sapiens 
2018-07-07 | E-MTAB-6984 | biostudies-arrayexpress
TranscriptomicsBulk RNA Barcoding and sequencing (BRB-seq) protocol for RNA-seq of 60 human LCL from the 1000G project
Here, we validate a novel protocol, Bulk RNA Barcoding and sequencing (BRB-seq), that combines the multiplexing-driven cost-effectiveness of a single-cell RNA-seq protocol with the efficiency of a bulk RNA-seq procedure. For this we use BRB-seq protocol on 60 human LCL data from the 1000G project sa...
ORGANISM(S): Homo sapiens 
2018-12-17 | E-MTAB-7524 | biostudies-arrayexpress
ProteomicsCOL1A2 Barcoding: Bone Species Identification via Shotgun Proteomics
Species identification of fragmentary bones remains a challenging task in archeology and forensics. A species identification method for such fragmentary bones that has recently attracted interest is the use of bone collagen proteins. We developed a method similar to DNA barcoding that reads collagen...
ORGANISM(S): Struthio camelus (Ostrich) Lithobates catesbeianus Anas platyrhynchos Mus musculus (Mouse) Cyprinus carpio (Common carp) Columba livia Thunnus albacares Sus scrofa domesticus (domestic pig) Seriola dumerili Oreochromis niloticus Cervus elaphus (Red deer) Coturnix japonica Phasianus colchicus Rattus norvegicus (Rat) Capra hircus (Goat) Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) Xiphias gladius Gallus gallus (Chicken) Paralichthys olivaceus Ovis aries Takifugu rubripes Numida meleagris Bos taurus (Bovine) Ursus arctos (Brown bear) (Grizzly bear) Salmo salar (Atlantic salmon) Oryctolagus cuniculus (Rabbit) Crocodylus porosus Thunnus maccoyii Pelodiscus sinensis Equus caballus (Horse) 
2023-12-14 | PXD045402 | Pride
Protein barcoding Species identification Sequest Col1a2 
TranscriptomicsDroplet barcoding for single cell transcriptomics applied to embryonic stem cells
Recently, RNA sequencing has achieved single cell resolution, but what is limiting is an effective way to routinely isolate and process large numbers of individual cells for in-depth sequencing, and to do so quantitatively. We have developed a droplet-microfluidic approach for parallel barcoding tho...
ORGANISM(S): Homo sapiens 
2015-05-20 | E-GEOD-65525 | biostudies-arrayexpress
GenomicsHaloTag-based conjugation of proteins to barcoding-oligonucleotides
HaloTag-based conjugation of proteins to barcoding-oligonucleotides
PRJNA505510 | ENA
ProteomicsIntact protein barcoding enables one-shot identification of CRISPRi strains and their metabolic state
Detecting strain-specific barcodes with mass spectrometry can facilitate the screening of genetically engineered bacterial libraries. Here, we introduce intact protein barcoding, a method to measure protein-based library barcodes and metabolites using flow-injection mass spectrometry (FI-MS). Protei...
ORGANISM(S): Escherichia Coli (ncbitaxon:562) 
2024-10-30 | MSV000096254 | MassIVE
UnknownMassively parallel assessment of designed protein solution properties using mass spectrometry and peptide barcoding.
Not available
S-EPMC11888366 | biostudies-literature
OtherHaloTag-based conjugation of proteins to barcoding-oligonucleotides
We describe the development of a high-sensitivity protein quantification system called HaloTag protein barcoding assay. The assay involves target protein linking to a unique molecule-counting oligonucleotide by click chemistry.
ORGANISM(S): synthetic construct 
2019-11-04 | GSE122542 | GEO
ProteomicsEvaluation of protein artifacts in the NicheProt workflow
This dataset contains proteomic analyses that validate the spatial proteomics technology, NicheProt. NicheProt is a 3D optical microscopy–guided, photobleaching-mediated cell barcoding approach designed to isolate intact cell types from specific microanatomical niches. We evaluate the proteomic prof...
ORGANISM(S): Mus musculus (Mouse) 
2026-03-19 | PXD075769 | Pride
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