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UnknownUsing SEQUEST with theoretically complete sequence databases.
Not available
S-EPMC4607654 | biostudies-literature
UnknownProLuCID: An improved SEQUEST-like algorithm with enhanced sensitivity and specificity.
Not available
S-EPMC4630125 | biostudies-literature
UnknownOptimization of filtering criterion for SEQUEST database searching to improve proteome coverage in shotgun proteomics.
Not available
S-EPMC2040164 | biostudies-literature
ProteomicsEvaluation of scoring functions and peptide exposure by fractionation
Evaluation of sensitivity and accuracy of widely used scoring functions Sequest, MaxQuant, Peaks, and Byonic. Spectra from E. coli were matched to human sequences, and human spectra from K052 cells were matched to A. loki sequences to determine specificity of matching and FDR estimation. A subsampli...
ORGANISM(S): Homo sapiens (Human) 
2018-10-18 | PXD008463 | Pride
Algorithm benchmark proteogenomics peaks sequest byonic maxquant 
ProteomicsErastin and ferrostatin induced protein expression change in HepG2 cells
Project description: HepG2 cells were treated with DMSO, erastin and ferrostatin, respectively. Protein was extracted and separated using SDS-PAGE electrophoresis. MS analysis was performed and protein was identified and quantified using Proteome Discoverer™ 1.3 software using the SEQUEST® search en...
ORGANISM(S): Homo sapiens (Human) 
2019-11-12 | PXD010761 | Pride
Erastin Ferroptosis Liver cancer 
ProteomicsA mass-tolerant database search identifies a large proportion of unassigned spectra in shotgun proteomics as modified peptides
Less than half of all MS/MS spectra acquired in shotgun proteomics typically result in a confident peptide match. Here we present an ultra-tolerant Sequest database search that allowed peptide matching even with modifications of unknown masses up to ±500 Da. From an HEK293 cell proteome-wide datase...
ORGANISM(S): Homo sapiens (Human) 
2015-06-03 | PXD001468 | Pride
Sequest Large scale Modifications Proteomics 
ProteomicsSecretome proteomic dataset of Filobasidium magnum PLANB_3024 cultured with poly(lactic acid)
This dataset contains bottom-up LC-MS/MS data from the culture-supernatant-derived extracellular protein fraction of Filobasidium magnum PLANB_3024 cultivated in the presence of poly(lactic acid) (PLA). Proteins were identified using Proteome Discoverer v3.0 with Sequest HT against a strain-specific...
ORGANISM(S): Filobasidium magnum 
2026-09-06 | PXD083739 | Pride
Pla Cutinase Filobasidium magnum Secretome 
MetabolomicsGNPS - Monsterscript_reverse_transcriptase_comparison
Tandem mass spectra were extracted, charge state deconvoluted and deisotoped by [unknown] version [unknown]. All MS/MS samples were analyzed using Sequest (XCorr Only) (Thermo Fisher Scientific, San Jose, CA, USA; version IseNode in Proteome Discoverer 2.2.0.388). Sequest (XCorr Only) was set up to ...
ORGANISM(S): Geobacillus Stearothermophilus (ncbitaxon:1422) 
2018-11-09 | MSV000083126 | GNPS
UnknownEnhanced peptide quantification using spectral count clustering and cluster abundance.
Not available
S-EPMC3234305 | biostudies-literature
ProteomicsErastin induced protein expression change in H1975 cells
H1975 cells were treated with DMSO and erastin, respectively. Protein was extracted and separated using SDS-PAGE electrophoresis. MS analysis was performed and protein was identified and quantified using Proteome Discoverer™ 1.3 software using the SEQUEST® search engine.
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD020205 | Pride
Erastin Ferroptosis Lung cancer 
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