Sort   by:  
 Page size 
ProteomicsLabeling of Bacillus subtilis and Escherichia coli to saturation with different percentages of fully 13C labeled glucose for benchmarking of protein-based stable isotope probing proteomics (Protein-SIP)
This dataset is part of a study aimed at developing algorithms for the quantification of stable isotope content in microorganisms after labeling them with stable isotope-labeled substrates. In this dataset Escherichia coli and Bacillus subtilis cultures were labeled with different percentages of ful...
ORGANISM(S): Bacillus subtilis subsp. subtilis Escherichia coli 
2022-12-30 | PXD023693 | Pride
13c Carbon 13 Stable isotope probing Stable isotope Protein-sip 1d-lc 
ProteomicsLabeling Escherichia coli to saturation with 2.5 % of 15N ammonium in two conditions (aerobic and anaerobic) for benchmarking of protein-based stable isotope probing proteomics (Protein-SIP)
This dataset is part of a study aimed at developing algorithms for the quantification of stable isotope content in microorganisms after labeling them with stable isotope-labeled substrates. For this dataset Escherichia coli cultures were labeled with 2.5 % of 15N-labeled NH4Cl and grown either anaer...
ORGANISM(S): Escherichia coli 
2022-02-16 | PXD024288 | Pride
Nitrogen 15 Stable isotope probing Stable isotope 15n Protein-sip 1d-lc Metaproteomics Lc-ms/ms 
ProteomicsLabeling of Bacillus subtilis and Escherichia coli to saturation with different percentages of 13C2 labeled glucose for benchmarking of protein-based stable isotope probing proteomics (Protein-SIP)
This dataset is part of a study aimed at developing algorithms for the quantification of stable isotope content in microorganisms after labeling them with stable isotope-labeled substrates. In this dataset Escherichia coli and Bacillus subtilis cultures were labeled with different percentages of sin...
ORGANISM(S): Escherichia coli Bacillus subtilis subsp. subtilis 6051-HGW 
2022-12-30 | PXD024285 | Pride
13c Carbon 13 Stable isotope probing Stable isotope Protein-sip 1d-lc Metaproteomics Lc-ms/ms 
ProteomicsMock microbial community with spike-ins of 13C-labeled Escherichia coli cells for benchmarking of protein-based stable isotope probing proteomics (Protein-SIP)
This dataset is part of a study aimed at developing algorithms for the quantification of stable isotope content in microorganisms in microbial communities after labeling them with stable isotope-labeled substrates. For this dataset Escherichia coli cultures were labeled with different percentages (1...
ORGANISM(S): Escherichia coli 
2022-12-30 | PXD024174 | Pride
13c Carbon 13 Stable isotope probing Stable isotope Protein-sip 1d-lc 
ProteomicsMixing of Escherichia coli cells labeled with 13C glucose with unlabeled E. coli cells for benchmarking of protein-based stable isotope probing proteomics (Protein-SIP)
This dataset is part of a study aimed at developing algorithms for the quantification of stable isotope content in microorganisms after labeling them with stable isotope-labeled substrates. In this dataset Escherichia coli cultures were labeled with different percentages (1% or 10%) of either single...
ORGANISM(S): Escherichia coli 
2022-12-30 | PXD024287 | Pride
13c Carbon 13 Stable isotope probing Stable isotope Protein-sip 1d-lc Metaproteomics Lc-ms/ms 
ProteomicsMetaProSIP protein-SIP data - MetaProSIP: automated inference of stable isotope incorporation rates in proteins for functional metaproteomics
MetaProSIP offers an automated high-throughput solution for 13C or 15N protein-SIP experiments. Labeled peptides are identified based on their unlabeled isotopologues. It calculates incorporation of heavy isotopes (RIA), the labeling ratio (LR) between proteins as well as additional shape properties...
ORGANISM(S): uncultured bacterium 
2014-11-27 | PXD000382 | Pride
Protein-sip Metaprosip 
Proteomics15N Limitation of detection in Pseudomonas fluorescens
The limit of 15N detection in proteins of Pseudomonas putida was studied by LC-MS/MS. Cells were grown in the presence of 0.1%15N to 10% 15N.The method of protein-based stable isotope probing (protein-SIP) has previously been shown to allow the modeling of carbon fluxes in microbial communities, dem...
ORGANISM(S): Pseudomonas fluorescens 
2013-04-09 | PXD000127 | Pride
Protein-sip 15n Lc-msms 
ProteomicsProteomic stable isotope probing (proteomic SIP) using 13C‑E. coli spike‑ins analyzed by DDA LC–MS/MS
This project applies proteomic stable isotope probing (proteomic SIP) to evaluate detection and quantification of 13C‑labeled E. coli peptides within a complex mouse fecal microbiome background. Proteins were extracted, digested, and quantified before preparing spike‑in mixtures. Mouse fecal peptide...
ORGANISM(S): gut metagenome 
2026-08-30 | PXD075589 | Pride
Proteomic sip  stable isotope probing  metaproteomics  13c labeling  e. coli spike‑in  mouse fecal microbiome  dda  orbitrap fusion 
Proteomics15N labeling of mouse microbiota
This study investigated the in vivo dynamics of the microbiome using metaproteomics coupled with protein-SIP in mice.
ORGANISM(S): gut metagenome 
2021-08-26 | PXD017451 | Pride
Microbiota Mouse 15n stable isotope labeling Metaproteomics 
ProteomicsProteomic Stable Isotope Probing of Cecal Microbiota Using U ¹³C Glucose Under Anaerobic Conditions
This study applies proteomic stable isotope probing (proteomic SIP) to profile microbial protein synthesis during anaerobic incubation with 100% U ¹³C glucose. Cecal microbiota were cultured in deoxygenated M9 medium supplemented with 0.3 g L⁻¹ sodium thioglycolate, with U ¹³C glucose. Following inc...
ORGANISM(S): gut metagenome 
2026-09-12 | PXD075725 | Pride
Proteomic sip  u ¹³c glucose  ¹³c labeling  anaerobic incubation  m9 medium  mouse cecal microbiota  metaproteomics  nanolc  orbitrap fusion  dda  fasp  sipros 5  mgnify microbiome database 
Sort   by:  
 Page size