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ProteomicsQuantitative proteomic comparison between wild type Col and acinus-2 pinin-1 mutant using 15N metabolic labeling
ACINUS and PININ genes regulate transcription and alternative splicing (Bi et al., 2021) and we hypothesize that some proteins should have altered expression in the double mutant. Therefore, MS experiments were designed to identify these altered proteins on a global scale using 15N metabolic labeli...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-02-04 | PXD030081 | Pride
Arabidopsis Acinus Quantification 15n metabolic labeling Protein prospector Pinin 
ProteomicsCombined 15N-Labelling and TandemMOAC Quantifies Phosphorylation of MAP Kinase Substrates Downstream of MKK7 in Arabidopsis
Reversible protein phosphorylation is a widespread posttranslational modification that plays a key role in eukaryotic signal transduction. Due to the large dynamic range of protein abundance, low stoichiometry and transient nature of protein phosphorylation, the detection and accurate quantification...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-01-02 | PXD008200 | Pride
Phosphoproteomics Tandem moac 15n labeling Mapk Mpk substrate 
ProteomicsQuantitative proteomic comparison between wild type Col and sec-5 mutant using 15N metabolic labeling
SECRET AGENT (SEC) is an O-GlcNAc transferase involved in O-GlcNAcylation of many proteins. However, sec-5 null allele mutant phenotype is very subtle. To examine if there are proteins that show altered expression in the sec-5 mutant compared to wild-type Col, we designed MS experiments to identify ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-02-04 | PXD030575 | Pride
Arabidopsis Quantification 15n metabolic labeling Protein prospector Secret agent (sec) 
ProteomicsRelative quantification of 15N labeled acinus-2 pinin-1 and 14N labeled Col through Data Dependent Acquisition to obtain peptide information for targeted Parallel Reaction Monitoring experiment
To examine the properties of 14N and 15N labeled peptides using Parallel Reaction Monitoring (PRM) on a high resolution, high accuracy mass spectrometer we first set up this DDA experiment. The purpose of this experiment was to choose peptides that are good candidates for targeted quantification. Th...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-06-09 | PXD030098 | Pride
Arabidopsis Quantification Data dependent acquisition 15n metabolic labeling Relative quantification Silac Protein prospector 
ProteomicsUsing targeted quantification (PRM) to quantify proteins between wild type Col and acinus-2 pinin-1 mutant with 15N metabolic labeling
ACINUS and PININ genes regulate transcription and alternative splicing (Bi et al., 2021) and we hypothesize that some proteins should have altered expression in the double mutant. Therefore, MS experiments were designed to identify these altered proteins on a global scale using 15N metabolic labeli...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-04-19 | PXD030096 | Pride
Arabidopsis Acinus Quantification 15n metabolic labeling Protein prospector Targeted quantification Pinin Parallel reaction monitoring (prm) 
Proteomics15N Limitation of detection in Pseudomonas fluorescens
The limit of 15N detection in proteins of Pseudomonas putida was studied by LC-MS/MS. Cells were grown in the presence of 0.1%15N to 10% 15N.The method of protein-based stable isotope probing (protein-SIP) has previously been shown to allow the modeling of carbon fluxes in microbial communities, dem...
ORGANISM(S): Pseudomonas fluorescens 
2013-04-09 | PXD000127 | Pride
Protein-sip 15n Lc-msms 
ProteomicsTo invesigate the role of autophagy in Arabidopsis root cellular protein turnover and proteostasis (15N Spike-in root)
Protein turnover analysis using partial 15N labelling and protein abundance analysis using 15N spike-in were coupled to investiage the role of autophagy in protein degradation and proteostasis. Our findings show that dysfuncional autophagy due to loss-of-function autophagy components ATG5 and 11 lea...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-07-12 | PXD010992 | Pride
Arabidopsis 15n labelling Protein turnover Autophagy 
Proteomics15N labeling of mouse microbiota
This study investigated the in vivo dynamics of the microbiome using metaproteomics coupled with protein-SIP in mice.
ORGANISM(S): gut metagenome 
2021-08-26 | PXD017451 | Pride
Microbiota Mouse 15n stable isotope labeling Metaproteomics 
ProteomicsTo invesigate the role of autophagy in Arabidopsis shoot cellular protein turnover and proteomstasis (15N Spike-in shoot)
Protein turnover analysis using partial 15N labelling and protein abundance analysis using 15N spike-in were coupled to investiage the role of autophagy in protein degradation and proteostasis. Our findings show that dysfuncional autophagy due to loss-of-function autophagy components ATG5 and 11 lea...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-10-13 | PXD010948 | Pride
Arabidopsis Protein turnover 15n spike-in Autophagy 
ProteomicsParallel Reaction Monitoring of 42 peptide pairs with 15N metabolic labeling
To examine the properties of 14N and 15N labeled peptides from 15 metabolic labeling samples, we set up targeted quantification using Parallel Reaction Monitoring (PRM) on a high resolution, high accuracy mass spectrometer. We first examined the co-elution of light and heavy peptide forms. Furthermo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-06-09 | PXD030112 | Pride
Arabidopsis Quantification 15n metabolic labeling Protein prospector Targeted quantification Parallel reaction monitoring (prm) 
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