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UnknownDiverse Redoxome Reactivity Profiles of Carbon Nucleophiles.
Targeted covalent inhibitors have emerged as a powerful approach in the drug discovery pipeline. Key to this process is the identification of signaling pathways (or receptors) specific to (or overexpressed in) disease cells. In this context, fragment-based ligand discovery (FBLD) has significantly e...
S-EPMC5898444 | biostudies-literature
ProteomicsShotgun proteomics experiments to identify covalent modification of proteins in HCT116 cells by a novel covalent ligand, CL16
By coupling activity-based protein profiling (ABPP) with covalent ligand screening experiment, we have identified a cysteine-reactive covalent ligand, CL16, which can bind strongly and selectively with RhoA in vitro. In this experiment, we performed shotgun proteomics to investigate protein targets ...
ORGANISM(S): Homo sapiens (Human) 
2025-08-30 | PXD053036 | Pride
Protein target identification of a cysteine-reactive covalent ligand In colorectal cancer hct116 cells Cl16 
ProteomicsAccelerating multiplexed profiling of protein-ligand interactions: high-throughput plate-based reactive cysteine profiling with minimal input
Chemoproteomics investigates small molecule-protein interactions and has made significant progress in recent years. Despite its vast potential, the proteome-wide profiling of reactive cysteine ligandability remains a formidable task to adapt for high throughput applications. This is primarily due to...
ORGANISM(S): Homo sapiens (Human) 
2023-12-24 | PXD044402 | Pride
Reactive cysteine Abpp Chemoproteomics High throughput Tmt 
ProteomicsNAIA for cysteine profiling in HepG2 cells, protein target identification of covalent ligand and studying cysteine oxidative modifications
New cysteine probes, N-acryloylindole-alkynes (NAIAs), have been developed. We have applied NAIA-5 for cysteine profiling in HepG2 cell lysates, and the results have been compared to the conventional cysteine probe iodoacetamide-alkyne (IAA). NAIA-5 allows identification of more ligandable Cys than ...
ORGANISM(S): Homo sapiens (Human) 
2023-06-14 | PXD041264 | Pride
Profiling ligandable cysteines and studying cysteine oxidative modifications in human liver cancer cells hepg2 by naia-5 using bruker timstof ms 
ProteomicsProteome-Wide Target Identification Using Reactive Metallo-Scaffolds: A Platform for Metallodrug Discovery
Metal complexes offer unique opportunities as scaffolds in chemical biology and drug discovery, with tuneable geometry, modular coordination environments, and structural features not readily accessible with organic molecules. Here, we introduce reactive metallo-scaffolds (r-mS) - a new class of cova...
ORGANISM(S): Homo sapiens (Human) 
2026-09-21 | PXD068320 | Pride
Reactive metalloscaffolds Dia-pasef Ia-dtb 
ProteomicsProtein target identification of CL16 in HCT116 cells by an alkyne probe, CL16-alkyne
By coupling activity-based protein profiling (ABPP) with covalent ligand screening experiment, we have identified a cysteine-reactive covalent ligand, CL16, which can bind strongly and selectively with RhoA in vitro. In this experiment, we investigated the protein targets of CL16 in colorectal cance...
ORGANISM(S): Homo sapiens (Human) 
2025-08-30 | PXD053041 | Pride
Lc-ms/ms experiment on hct116 cells probed by cl16-alkyne to investigate protein targets of cl16 
ProteomicsPrioritization of antimicrobial targets by CRISPR-based oligo recombineering
Nucleophilic amino acids are important in covalent drug development yet underutilized as antimicrobial targets. Over recent years, several chemoproteomic technologies have been developed to mine chemically-accessible residues via their intrinsic reactivity toward electrophilic probes. However, these...
ORGANISM(S): Toxoplasma gondii RH 
2022-10-24 | PXD035658 | Pride
Toxoplasma gondii Oligo recombineering Proteomics 
ProteomicsMass spectrometric analyses of the oxidation state of Cys281 in peptide fragment.
Oxidized cysteine residues are highly reactive and can form functional covalent conjugates, of which the allosteric redox switch formed by the lysine-cysteine NOS bridge is an example. Here, we report a non-canonical FAD-dependent enzyme Orf1 that adds a glycine-derived N-formimidoyl group to glycin...
ORGANISM(S): Escherichia coli 
2023-05-10 | PXD041105 | Pride
N-formimmidolation and iminoacetylation enzyme 
ProteomicsMass spectrometric analyses for the C281-dimedone adduct and the sulfinylated peptide fragments of Orf1
Oxidized cysteine residues are highly reactive and can form functional covalent conjugates, of which the allosteric redox switch formed by the lysine-cysteine NOS bridge is an example. Here, we report a non-canonical FAD-dependent enzyme Orf1 that adds a glycine-derived N-formimidoyl group to glycin...
ORGANISM(S): Escherichia coli 
2023-05-10 | PXD041104 | Pride
N-formimmidolation and iminoacetylation enzyme 
ProteomicsThe sulfonic-containing peptides (at C281) from trypsin-treated Orf1 in H218O.
Oxidized cysteine residues are highly reactive and can form functional covalent conjugates, of which the allosteric redox switch formed by the lysine-cysteine NOS bridge is an example. Here, we report a non-canonical FAD-dependent enzyme Orf1 that adds a glycine-derived N-formimidoyl group to glycin...
ORGANISM(S): Escherichia coli 
2023-05-10 | PXD041103 | Pride
N-formimmidolation and iminoacetylation enzyme 
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