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ProteomicsDiscovery and optimisation of a covalent ligand for TRIM25 and its application to targeted protein ubiquitination
The tripartite motif (TRIM) family of RING-type E3 ligases catalyses the formation of many different types of ubiquitin chains, and as such, plays important roles in diverse cellular functions, ranging from immune regulation to cancer signalling pathways. Few ligands have been discovered for TRIM E3...
ORGANISM(S): Homo sapiens (Human) 
2025-05-19 | PXD061182 | Pride
Covalent ligand E3 ligase Ubiquitin Ia-dtb 
ProteomicsRobust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - Library Build, concentration response, structure activity relationships and in-cell treatment
In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. Within this workflow, we profile cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiob...
ORGANISM(S): Homo sapiens (Human) 
2024-11-18 | PXD054145 | Pride
Chemoproteomics. lavel-free quantification Data independant acquisition 
ProteomicsRobust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - 80 compound screen (HEK293T and Jurkat)
In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. Within this workflow, we profile cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiob...
ORGANISM(S): Homo sapiens (Human) 
2024-11-18 | PXD054127 | Pride
Chemoproteomics Data independant acquisition Label-free qauntification 
ProteomicsProteome-Wide Target Identification Using Reactive Metallo-Scaffolds: A Platform for Metallodrug Discovery
Metal complexes offer unique opportunities as scaffolds in chemical biology and drug discovery, with tuneable geometry, modular coordination environments, and structural features not readily accessible with organic molecules. Here, we introduce reactive metallo-scaffolds (r-mS) - a new class of cova...
ORGANISM(S): Homo sapiens (Human) 
2026-09-21 | PXD068320 | Pride
Reactive metalloscaffolds Dia-pasef Ia-dtb 
ProteomicsEnantioselective OTUD7B fragment discovery through chemoproteomics screening and high-throughput optimisation
Deubiquitinating enzymes (DUBs) are key regulators of cellular homeostasis, and their dysregulation is associated with several human diseases. The ovarian tumour protease (OTU) family of DUBs are biochemically well-characterised and of therapeutic interest, yet only a few tool compounds exist to stu...
ORGANISM(S): Homo sapiens (Human) 
2025-01-13 | PXD057851 | Pride
Dubs Dia Iodoacetamide-desthiobiotin (ia-dtb) Chemoproteomics Cysteine reactivity profiling Abpp 
ProteomicsCovalent fragment screening to inhibit the E3 ligase activity of bacterial NEL enzymes SspH1 and SspH2
As the global fight against antimicrobial resistance in bacteria becomes increasingly pressing, new tool compounds are needed to understand and identify novel therapeutic targets. Here, cysteine-directed fragment-based drug discovery is coupled with a high throughput chemistry direct-to-biology scre...
ORGANISM(S): Homo sapiens (Human) 
2025-11-10 | PXD057304 | Pride
Bacterial nels Fragment-based drug discovery Covalent fragments Salmonella effectors Chemoproteomics E3 ligase High throughput chemistry 
ProteomicsChemoproteomic Profiling of VVD-065 Selectivity
The NRF2 transcription factor is constitutively active in cancer where it functions to maintain oxidative homeostasis and reprogram cellular metabolism. NRF2-active tumors exhibit NRF2-dependency and resistance to chemo/radiotherapy. Here we characterize VVD-065, a first-in-class NRF2 inhibitor that...
ORGANISM(S): Homo sapiens (Human) 
2025-12-10 | PXD071555 | Pride
Nrf2 Keap1 Vvd-065 
ProteomicsChemoproteomic Cysteine Selectivity of VVD-065, VVD-330 and VVD-325
Global assessment of cysteine selectivity for VVD compounds that covalently engage KEAP1 C151
ORGANISM(S): Homo sapiens (Human) 
2025-12-10 | PXD071657 | Pride
Nrf2 Keap1 Vvd-330 Vvd-065 Vvd-325 
ProteomicsChemoproteomic Cysteine Selectivity of VVD-446 and VVD-860
Global assessment of cysteine selectivity for VVD compounds that covalently engage KEAP1 C151
ORGANISM(S): Homo sapiens (Human) 
2025-12-10 | PXD071609 | Pride
Vvd-860 Keap1 Vvd-446 
ProteomicsRobust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - HEK293T - Global expression proteomics
In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. This platform profiles cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiobiotin (IA-...
ORGANISM(S): Homo sapiens (Human) 
2024-11-18 | PXD054105 | Pride
Data independant acquistion Label free quantification 
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