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ProteomicsSelection of Heating Temperatures Improves the Sensitivity of Proteome Integral Solubility Alteration (PISA) Assay
The thermal shift assay is a robust method of discovering protein-ligand interactions by measuring the alterations in protein thermal stability under various conditions. Several thermal shift assays have been developed and their throughput has been advanced greatly by the rapid progress in tandem ma...
ORGANISM(S): Homo sapiens (Human) 
2020-04-06 | PXD017346 | Pride
Proteome integral solubility alteration (pisa) assay Human cell line lysate Lc-ms/ms 
ProteomicsProteome Integral Solubility Alteration (PISA): a high-throughput proteomics assay for target deconvolution
Various agents, including drugs as well as non-molecular influences, induce alterations in the physic-chemical properties of proteins in cell lysates, living cells and organisms. These alterations can be probed by applying a stability-modifying factor, such as elevated temperature, to a varying degr...
ORGANISM(S): Homo sapiens (Human) 
2020-04-01 | PXD013182 | Pride
Protein stability Target deconvolution Tmt Thermal profiling Quantitative proteomics 
UnknownSelection of Heating Temperatures Improves the Sensitivity of the Proteome Integral Solubility Alteration Assay.
Not available
S-EPMC7294736 | biostudies-literature
ProteomicsS. elongatus PCC 7942 Proteome Integral Solubility Alteration (PISA) Proteomics - Light vs Dark with Dense and Dilute
PISA experimental assays investigating structural alterations in Light vs Dark growth conditions with Dense and Dilute conditions. Samples are representative of Synechococcus elongatus PCC 7942 cell lysates (BTO:0004304). Purpose: Detecting structural and abundance changes in cyanobacterial cultures...
ORGANISM(S): Synechococcus Elongatus Pcc 7942 (ncbitaxon:1140) 
2025-11-14 | MSV000099914 | MassIVE
UnknownMerging multi-omics with proteome integral solubility alteration unveils antibiotic mode of action.
Not available
S-EPMC11434622 | biostudies-literature
ProteomicsDrPISA: Deep Eutectic Solvent-Assisted Reverse Proteome-Integrated Solubility Alteration for High-Sensitivity Drug Target Identification
Existing thermal shift-based mass spectrometry approaches, such as TPP and PISA, focus mainly on soluble fractions, often neglecting the insoluble pellet, limiting comprehensive drug–target profiling. In this work, we developed DrPISA (deep eutectic solvent-assisted reverse PISA), a novel strategy t...
ORGANISM(S): Homo Sapiens 
2025-07-14 | PXD066145 |
ProteomicsHepatocyte thorns, a novel drug-induced stress response in human and mouse liver spheroids
The in vivo-relevant phenotype of 3D liver spheroids allows for long-term studies of e.g. novel mechanisms of chronic drug-induced liver toxicity. Using this system, we present a novel drug-induced stress response in human and murine hepatocyte spheroids wherein long slender filaments form after chr...
ORGANISM(S): Homo sapiens (Human) 
2022-05-26 | PXD033550 | Pride
Hepatocytes  spheroids  thorns  hepatotoxicity  keratins  3d culture 
ProteomicsPISA-based thermal proteome profiling of human liver tissue exposed to environmental pollutants
This study applied the Proteome Integral Solubility Alteration (PISA) approach combined with DIA-MS to characterize protein stability changes in human liver tissue exposed to environmental pollutants, including BPA, PAH, PFOS, and TMP. The work provides a proteome-wide overview of pollutant-induced ...
ORGANISM(S): Homo Sapiens 
2025-11-03 | PXD070215 |
ProteomicsReprogramming SREBP1-dependent lipogenesis and inflammation in high-risk breast with licochalcone A: a novel path to cancer prevention
This study investigates the effects of Licochalcone A (LicA) on protein stability in breast cancer cells using Proteome Integral Solubility Alterations (PISA) proteomics. We treated MCF-7 (ER+) and MDA-MB-231 (ER-) cells with LicA (10 µM) for 24 hours and subjected them to temperature-dependent prot...
ORGANISM(S): Homo sapiens (Human) 
2025-09-29 | PXD061086 | Pride
Proteomics Licochalcone a Lipid metabolism Breast cancer prevention Lc-ms Srebp Antiproliferation Proteome integral solubility alterations (pisa) Pi3k-akt pathway Metabolic reprogramming Anti-inflammation 
TranscriptomicsMitochondrial L-2-hydroxyglutarate is a physiologic signaling metabolite [scRNA-seq]
L-2-hydroxyglutarate (L-2-HG) is a low-abundance metabolite in mammals due to the mitochondrial enzyme L-2-HG dehydrogenase (L2HGDH), which oxidizes L-2-HG to 2-oxoglutarate (2-OG) to prevent its accumulation. In humans lacking L2HGDH activity, L-2-HG builds up, leading to L-2-hydroxyglutaric acidur...
ORGANISM(S): Mus musculus 
2026-04-08 | GSE326782 | GEO
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