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ProteomicsA ‘proteomic ruler’ for protein copy number and concentration estimation without spike-in standards
Absolute protein quantification using mass spectrometry (MS)-based proteomics delivers protein concentrations or copy numbers per cell. Existing methodologies typically require a combination of isotope-labeled spike-in references, cell counting and protein concentration measurements. Here we present...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2014-09-17 | PXD000661 | Pride
Copy numbers Absolute quantification 
UnknownA "proteomic ruler" for protein copy number and concentration estimation without spike-in standards.
Not available
S-EPMC4256500 | biostudies-literature
OtherA proteomic ruler for protein copy number and concentration estimation without spike-in standards.
Data from ProteomeXchange, PXD ID: PXD000661. Experiment: A549, file: 20121023_EXQ4_JRW_SA_32L_3.mzml. Published as part of Mol Cell Proteomics. 2014 Sep 15 . From the Abstract: {{i}} ... Here we present a novel concept that delivers similar quantitative results directly from deep eukaryotic proteo...
ORGANISM(S): Homo_sapiens_viruses, Human 
GPM32320003525 | GPMDB
Resource Reanalysis 
OtherA proteomic ruler for protein copy number and concentration estimation without spike-in standards.
Data from ProteomeXchange, PXD ID: PXD000661. Experiment: TPA_Lung, file: 20120207_EXQ6_JRW_TPA_Lung_28_1_T.mzml. Published as part of Mol Cell Proteomics. 2014 Sep 15 . From the Abstract: {{i}} ... Here we present a novel concept that delivers similar quantitative results directly from deep eukary...
ORGANISM(S): Mus_musculus_viruses, Mouse 
GPM11210029853 | GPMDB
Resource Reanalysis 
ProteomicsThe yeast peroxisomal proteome at absolute quantitative scale
Peroxisomes are dynamic organelles vital for lipid metabolism and redox homeostasis. In Saccharomyces cerevisiae, the expression of peroxisomal proteins is tightly regulated in response to metabolic conditions. Here, we provide the first absolute quantification of the yeast peroxisomal proteome unde...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2026-02-16 | PXD069526 | Pride
Saccharomyces cerevisiae  peroxisomes  protein copy numbers  absolute quantification  proteomic ruler  mass spectrometry 
ProteomicsComprehensive quantitative analysis of the proteome of Leishmania (Viannia) species
Leishmania species are responsible for a broad spectrum of diseases, denominated Leishmaniasis, affecting over 12 million people worldwide. During the last decade, there have been impressive efforts for sequencing the genome of most of the pathogenic Leishmania spp. as well as hundreds of strains, b...
ORGANISM(S): Leishmania guyanensis species complex 
2020-08-10 | PXD017696 | Pride
Leishmania (viannia)  leishmania braziliensis  leishmania panamensis  leishmania guyanensis  proteomics  label-free quantification  filter aided sample preparation Proteomic ruler Total protein approach Mass spectrometry 
ProteomicsImpact of collagenase treatment on the proteome and transcriptome of human articular chondrocytes
Objective Human primary articular chondrocytes (hPACs) are routinely isolated from articular cartilage and for pre-clinical OA research. Collagenase digest of tissue is an essential step, yet the impact of enzymatic incubation on the phenotype of hPACs is unclear. We aim to delineate this through p...
ORGANISM(S): Homo sapiens (Human) 
2025-10-20 | PXD064948 | Pride
Collagenase Chondrocytes Osteoarthritis Transcriptomic Proteomic Phenotype 
ProteomicsProtein abundance of AKT and ERK pathway components governs cell-type-specific regulation of proliferation
Signaling through the AKT and ERK pathways controls cell proliferation. However, the integrated regulation of this multistep process, involving signal processing, cell growth and cell-cycle progression, is poorly understood. Here we study different murine hematopoietic cell types, in which AKT and E...
ORGANISM(S): Homo sapiens (Human) 
2017-01-30 | PXD004816 | Pride
Human Cfu-e Proteome ruler Label-free quantification 
ProteomicsComparison of the Cowpox Virus and Vaccinia Virus Mature Virion Proteome: Analysis of the species- and strain-specific Proteome
Cowpox virus (CPXV) causes most zoonotic orthopoxvirus (OPV) infections in Europe and Northern as well as Central Asia. The virus has the broadest host range of OPV and is transmitted to humans from rodents and other wild or domestic animals. Increasing numbers of human CPXV infections in a populati...
ORGANISM(S): Orthopoxvirus 
2015-11-16 | PXD003013 | Pride
Ltq-orbitrap Virion proteome Orthopoxvirus 
ProteomicsProteome Profiling of PBMC-originated, iPSC-derived Lentoid Bodies at differentiation day 35
Differentiation of pluripotent stem cells into lentoid bodies is important for the understanding of the lens development and investigating the processes critical for lens morphogenesis. This Study was initiated to investigate a comprehensive proteome profiling of the peripheral blood mononuclear cel...
ORGANISM(S): Homo sapiens (Human) 
2022-02-28 | PXD009633 | Pride
Mass-spectrometry Ipsc-derived lentoid bodies Proteome Pbmc 
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