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ProteomicsControl of TurboID-dependent biotinylation intensity in proximity ligation screens
Proximity biotinylation screens are a widely used strategy for the unbiased identification of interacting or vicinal proteins. The latest generation biotin ligase TurboID has broadened the range of potential applications, as this ligase promotes an intense and faster biotinylation, even in subcellul...
ORGANISM(S): Homo sapiens (Human) 
2023-03-29 | PXD040302 | Pride
Biotinylation Proximity ligation screens Protein-protein interactions Proteomics 
ProteomicsProximity-dependent biotinylation by TurboID to identify protein-protein interaction networks in yeast
The use of proximity-dependent biotinylation assays coupled to mass spectrometry (PDB-MS) has changed the field of protein-protein interactions (PPI) studies. Yet, despite the recurrent and successful use of BioID-based PPI screening in mammalian cells, the implementation of PDB-MS in yeast has not ...
ORGANISM(S): Schizosaccharomyces pombe 927 
2019-05-16 | PXD013183 | Pride
Proximity-dependent biotinylation  turboid  yeast  s. pombe  rmt3 Rna exosome 
UnknownOptical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells.
Not available
S-EPMC8890125 | biostudies-literature
ProteomicsProximity-dependent biotinylation to elucidate the interactome of TNK2 non-receptor tyrosine kinase
Non-receptor tyrosine kinases represent an important class of signaling molecules which are involved in driving diverse cellular pathways. Although the large majority have been well-studied in terms of their protein binding partners, the interactomes of some important non-receptor tyrosine kinases s...
ORGANISM(S): Homo sapiens (Human) 
2021-08-25 | PXD010474 | Pride
Tnk2 Human Bioid Biosite 
ProteomicsBIOSITe: A novel method for direct detection of site specific biotinylation
Proximity-dependent biotinylation methods, such as those mediated by BioID and APEX, are being widely adopted for studying in vivo protein-protein interaction dynamics and subcellular structures. However, these along with other biotin-based technologies are severely dependent on avidin family protei...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2017-12-19 | PXD007862 | Pride
Bioid Proximity dependent biotinylation Bcr abl Mass spectrometry Ip Peptide Biotinylation Biosite 
UnknownA Versatile Lentiviral Delivery Toolkit for Proximity-dependent Biotinylation in Diverse Cell Types.
Not available
S-EPMC6210231 | biostudies-literature
ProteomicsDefinition of the fibroblast adhesome using multiplexed proximity biotinylation
Integrin adhesion complexes (IACs) bridge the extracellular matrix to the actin cytoskeleton and transduce signals in response to both chemical and mechanical cues. The composition, interactions, stoichiometry and topological organisation of proteins within IACs are not fully understood. To address ...
ORGANISM(S): Mus musculus (Mouse) 
2020-06-01 | PXD017241 | Pride
Fibroblast Adhesome Bioid 
UnknownScreening of Proximal and Interacting Proteins in Rice Protoplasts by Proximity-Dependent Biotinylation.
Not available
S-EPMC5427108 | biostudies-literature
ProteomicsANXA6 proximity-dependent biotinylation assay using ultraID in HeLa cells
Analysis of ANXA6 proximity interactome using ultraID (birA* derived biotin ligase) in HeLa ANXA6ko cells. We performed a proximity-dependent biotinylation assay (UltraID) followed by streptavidin enrichment using Dynabeads and LC-MS/MS.
ORGANISM(S): Homo sapiens (Human) 
2026-06-29 | PXD078364 | Pride
Hela cells Ultraid Proximity labeling Anxa6 Biotinylation Interactome 
ProteomicsConfirmation of replication interactome of TopBP1 using proximity-dependent biotinylation MS
Goal of the Experiment is to confirm the replication interactome of TopBP1 using stabile 293 FlpIn cell lines expressing TopBP1 adapter fused to APEX2. Proteins in its close proximity will become biotinylated by APEX2, thereby enabling the pulldown of biotinylated proteins and thus, replication invo...
ORGANISM(S): Homo sapiens (Human) 
2024-05-21 | PXD040000 | Pride
Topbp1 Proximity-dependent biotinylation 
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