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GenomicsAliirhizobium smilacinae strain:PTYR-5
Aliirhizobium smilacinae strain:PTYR-5 Genome sequencing and assembly
PRJNA546479 | ENA
ProteomicsQuantative pTyr protein complex
Our current research focuses on the complex-centric quantification of pTyr protein complexes. We initially constructed a tri-functional probe based on pTyr superbinder (SH2-S) for the enrichment of pTyr protein complexes. The pTyr protein complexes were separated by ion exchange chromatography (IEC)...
ORGANISM(S): Homo Sapiens 
PXD052742 |
ProteomicsQuantitative consequences of protein carriers in immunopeptidomics and tyrosine phosphorylation MS2 analyses
Evaluating the impact of a carrier proteome on data quality and quantity in multiplexed pTyr and MHC analyses.
ORGANISM(S): Homo sapiens (Human) 
2021-06-01 | PXD025073 | Pride
Mhc Phosphorylation Lc-ms/ms 
ProteomicsA sensitive and cost-effective approach for tyrosine phosphoproteome analysis based on SH2 superbinder
Albeit much less abundant than Ser/Thr phosphorylation (pSer/pThr), Tyr phosphorylation (pTyr) is considered a hallmark in cellular signal transduction. However, its analysis remains a challenge. The conventional immunopurification (IP) approach using antibodies pan-specific to pTyr sites is known t...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2017-08-29 | PXD005838 | Pride
Lc ms/ms Sh2 superbinder Ptyr phosphoproteome 
UnknownExploring the Existing Drug Space for Novel pTyr Mimetic and SHP2 Inhibitors.
Not available
S-EPMC4499873 | biostudies-literature
ProteomicsAlteration in Tyrosine Phosphorylation of Cardiac Proteome and EGFR Pathway Contribute to Hypertrophic Cardiomyopathy
Alterations of serine/threonine phosphorylation of the cardiac proteome are a hallmark of heart failure. However, the contribution of tyrosine phosphorylation (pTyr) to the pathogenesis of cardiac hypertrophy remains unclear. We use global mapping to discover and quantify site-specific pTyr in two c...
ORGANISM(S): Mus musculus (Mouse) 
2023-03-10 | PXD036506 | Pride
Heart Tyrosine phosphorylation Proteomics Serine phosphorylation Threonine phosphorylation 
Proteomics[HPP-2.0] Integrated and high-throughput approach for sensitive analysis of tyrosine phosphoproteome
In this study, we developed a 96-well microplate-based approach to integrate all the sample preparation steps starting from cell culture to MS-compatible pTyr peptide enrichment in three consecutive 96-well microplates. By assembling engineered SH2 domain onto microplate, non-specific adsorption of ...
ORGANISM(S): Homo Sapiens 
2024-02-03 | PXD049140 |
ProteomicsIdentification of phosphotyrosine (pTyr)-containing T cell proteins
This project aimed at identifying phosphotyrosine (pTyr)-containing T cell proteins that bind directly to the pTyr-binding pocket in the N-terminal C2 domain of protein kinase C-theta. We used an immobilized recombinant GST-C2 fusion protein to pull-down proteins present in lysates of stimulated hum...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-03-13 | MSV000083562 | MassIVE
ProteomicsPhosphoproteomics scaled-down to tumor biopsy level. Part 2: biopsies
Mass spectrometry-based phosphoproteomics of tumor tissue or cell line lysates provides insight in aberrantly activated signaling pathways and potential drug targets. For improved understanding of the individual patient’s tumor biology, analysis of the phosphoproteome should be feasible and reproduc...
ORGANISM(S): Homo sapiens (Human) 
2019-10-28 | PXD003814 | Pride
Human Biopsy Single-shot Ptyr Crc Label-free Phosphoproteomics Colorectal cancer 
ProteomicsPhosphoproteomics scaled-down to tumor biopsy level -part 1 cell line
Mass spectrometry-based phosphoproteomics of tumor tissue or cell line lysates provides insight in aberrantly activated signaling pathways and potential drug targets. For improved understanding of the individual patient’s tumor biology, analysis of the phosphoproteome should be feasible and reproduc...
ORGANISM(S): Homo sapiens (Human) 
2022-03-02 | PXD003808 | Pride
Human Biopsy Single-shot Ptyr Crc Label-free Phosphoproteomics Colorectal cancer 
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