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The yeast RNA binding protein PUB1 contains a prion-like domain (PrLD) and, when overexpressed, localizes into cytoplasmic liquid droplets and is toxic. However, truncated versions lacking the PrLD are either soluble or aggregating and do not significantly alter yeast growth. Analysis of the interac...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-02-04 | PXD011887 | Pride
RNA Sequencing and Ribosome profiling of GIR2 and PUB1 knockout yeast strains.
We present ribosome profiling and RNA-Seq data for the wild type BY4742 S. cerevisiae strain, and strains with the complete individual deletions of the GIR2 and PUB1 genes.
ORGANISM(S): Saccharomyces cerevisiae 
2021-10-11 | GSE185286 | GEO
We investigated the sub-cellular architecture of Stress Granules in the context of glucose starvation. In order to uncover transient and peripheral Stress Granule interactors, we optimized the proximity-dependent biotinylation (BioID) approach for use in yeast. This approach allowed us to visualize ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-23 | PXD016988 | Pride
We analyzed the effect of PUB1 and NGR1 on the transcription rates, mRNA stabilities and mRNA levels by doing GRO experiments in a deletion mutants comparing with a wild type in both glucose (YPD) and galactose (YPGal) media. Some data for the wt in YPGal are not included because were already in GEO...
ORGANISM(S): Saccharomyces cerevisiae 
2015-12-31 | GSE63729 | GEO
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